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Molecular detection of virulence factors among food and clinical Enterococcus faecalis strains in South Brazil

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Molecular detection of virulence factors among food and clinical Enterococcus faecalis strains in South Brazil

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Título Molecular detection of virulence factors among food and clinical Enterococcus faecalis strains in South Brazil
Autor Medeiros, Aline Weber
Pereira, Rebeca Inhoque
Oliveira, Daniele Vargas de
Martins, Paula Dalcin
D'Azevedo, Pedro Alves
Van der Sand, Sueli Terezinha
Frazzon, Jeverson
Frazzon, Ana Paula Guedes
Abstract The present report aimed to perform a molecular epidemiological survey by investigating the presence of virulence factors in E. faecalis isolated from different human clinical (n = 57) and food samples (n = 55) in Porto Alegre, Brazil, collected from 2006 to 2009. In addition, the ability to form biofilm in vitro on polystyrene and the -haemolytic and gelatinase activities were determined. Clinical strains presented a higher prevalence of aggregation substance (agg), enterococcal surface protein (esp) and cytolysin (cylA) genes when compared with food isolates. The esp gene was found only in clinical strains. On the other hand, the gelatinase (gelE) and adherence factor (ace) genes had similar prevalence among the strains, showing the widespread occurrence of these virulence factors among food and clinical E. faecalis strains in South Brazil. More than three virulence factor genes were detected in 77.2% and 18.2% of clinical and food strains, respectively. Gelatinase and -haemolysin activities were not associated with the presence of gelE and cylA genes. The ability to produce biofilm was detected in 100% of clinical and 94.6% of food isolates, and clinical strains were more able to form biofilm than the food isolates (Student’s t-test, p < 0.01). Results from the statistical analysis showed significant associations between strong biofilm formation and ace (p = 0.015) and gelE (p = 0.007) genes in clinical strains. In conclusion, our data indicate that E. faecalis strains isolated from clinical and food samples possess distinctive patterns of virulence factors, with a larger number of genes that encode virulence factors detected in clinical strains.
Contido em Brazilian journal of microbiology. São Paulo. Vol. 45, no. 1 (Jan./Mar. 2014), p. 327-332
Assunto Biofilme
Enterococcus faecalis
Virulência
[en] Biofilm formation
[en] Clinical enterococci
[en] Food enterococci
[en] virulence determinants
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/100121
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