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Cell block technique as an additional tool in the diagnosis of ameloblastoma

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Cell block technique as an additional tool in the diagnosis of ameloblastoma

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Título Cell block technique as an additional tool in the diagnosis of ameloblastoma
Autor Belatto, Maria Fernanda da Silva
Ramos, Grasieli de Oliveira
Martins, Manoela Domingues
Grando, Liliane Janete
Rivero, Helena Riet Correa
Abstract The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stained with haematoxylin and eosin (H&E). Immunohistochemical reactions against anti-pan-cytokeratin (AE1/AE3) were performed to measure the presence of epithelial cells. Cytological analyses of the obtained slides revealed the presence of epithelial cells (as evidenced by AE1/ AE3 labelling) and acellular amorphous eosinophilic materials. These cytological findings, in light of clinical and imaging data, can be helpful in the presumptive diagnosis of this disease entity by eliminating other possible diagnoses. Nonetheless further studies are needed in order to determine the nature of the acellular amorphous eosinophilic material and to ascertain the immunoprofile of epithelial cells.
Contido em Brazilian oral research. São Paulo. Vol. 28, no. 1 (Jan. 2014), p. 1-6
Assunto Ameloblastoma
Biópsia por agulha fina
Tumores : Odontogenicos
[en] Ameloblastoma
[en] Biopsy
[en] Cell biology
[en] Fine-needle
[en] Odontogenic tumors
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/106722
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