Repositório Digital

A- A A+

Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey

.

Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey

Mostrar registro completo

Estatísticas

Título Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
Autor Rech, Rosane
Ayub, Marco Antônio Záchia
Abstract Saccharomyces cerevisiae strain W303 was transformed with two yeast integrative plasmids containing Kluyveromyces lactis LAC4 and LAC12 genes that codify β-galactosidase and lactose permease respectively. The BLR030 recombinant strain was selected due to its growth and β-galactosidase production capacity. Different culture media based on deproteinized cheese whey (DCW) were tested and the best composition (containing DCW, supplemented with yeast extract 1 %, and peptone 3 % (w/v)) was chosen for bioreactor experiments. Batch, and fed-batch cultures with linear ascending feeding for 25 (FB25), 35 (FB35), and 50 (FB50) hours, were performed. FB35 and FB50 produced the highest β-galactosidase specific activities (around 1,800 U/g cells), and also the best productivities (180 U/L.h). Results show the potential use of fed-batch cultures of recombinant S. cerevisiae on industrial applications using supplemented whey as substrate.
Contido em Brazilian Journal of Chemical Engineering. São Paulo. vol. 23, n. 4 (oct./dec. 2006), p. 435-442
Assunto beta-Galactosidase
Saccharomyces cerevisiae recombinante
Soro de leite
[en] Cheese-whey
[en] Fed-batch cultivation
[en] Recombinant Saccharomyces cerevisiae
[en] β-galactosidase
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/107573
Arquivos Descrição Formato
000567377.pdf (230.9Kb) Texto completo (inglês) Adobe PDF Visualizar/abrir

Este item está licenciado na Creative Commons License

Este item aparece na(s) seguinte(s) coleção(ões)


Mostrar registro completo

Percorrer



  • O autor é titular dos direitos autorais dos documentos disponíveis neste repositório e é vedada, nos termos da lei, a comercialização de qualquer espécie sem sua autorização prévia.
    Projeto gráfico elaborado pelo Caixola - Clube de Criação Fabico/UFRGS Powered by DSpace software, Version 1.8.1.