Repositório Digital

A- A A+

Testis-mediated gene transfer in mice : comparison of transfection reagents regarding transgene transmission and testicular damage

.

Testis-mediated gene transfer in mice : comparison of transfection reagents regarding transgene transmission and testicular damage

Mostrar registro completo

Estatísticas

Título Testis-mediated gene transfer in mice : comparison of transfection reagents regarding transgene transmission and testicular damage
Autor Amaral, Marta G.
Campos, Vinicius Farias
Seixas, Fabiana Kömmling
Cavalcanti, Paulo Varoni
Selau, Lisiane Priscila Roldão
Deschamps, João Carlos
Collares, Tiago Veiras
Abstract Testis-mediated gene transfer (TMGT) has been used as in vivo gene transfer technology to introduce foreign DNA directly into testes, allowing mass gene transfer to offspring via mating. In this study, we used plasmid DNA (pEGFP-N1) mixed with dimethylsulfoxide (DMSO), N,N-dimethylacetamide (DMA) or liposome (Lipofectin) in an attempt to improve TMGT. Males receiving consecutive DNA complex injections were mated to normal females to obtain F0 progeny. In vivo evaluation of EGFP expression, RT-PCR and PCR were used to detect the expression and the presence of exogenous DNA in the progeny. We also evaluated possible testicular damage by histological procedures. PCR and RT-PCR analyses revealed that liposome and DMSO increased the rate of TMGT. Histological analyses demonstrated that repeated (4 times) injections of DNA complexes can affect spermatogenesis. DMSO was the most deleterious among the reagents tested. In this study, we detected the presence of transgene in the progeny, and its expression in blood cells. Consecutive injections of DNA complexes were associated with impaired spermatogenesis, suggesting requirement of optimal conditions for DNA delivery through TMGT.
Contido em Biological research. Santiago. Vol. 44, no. 3 (2011), p. 224-234.
Assunto Estatística médica
[en] DMA (N,N-dimethylacetamide)
[en] Histological damage
[en] Liposome
[en] Mice
[en] TMGT
[en] Transgenesis
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/108645
Arquivos Descrição Formato
000870834.pdf (1.324Mb) Texto completo (inglês) Adobe PDF Visualizar/abrir

Este item está licenciado na Creative Commons License

Este item aparece na(s) seguinte(s) coleção(ões)


Mostrar registro completo

Percorrer



  • O autor é titular dos direitos autorais dos documentos disponíveis neste repositório e é vedada, nos termos da lei, a comercialização de qualquer espécie sem sua autorização prévia.
    Projeto gráfico elaborado pelo Caixola - Clube de Criação Fabico/UFRGS Powered by DSpace software, Version 1.8.1.