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Lipopolysaccharide modulates astrocytic S100B secretion: a study in cerebrospinal fluid and astrocyte cultures from rats

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Lipopolysaccharide modulates astrocytic S100B secretion: a study in cerebrospinal fluid and astrocyte cultures from rats

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Título Lipopolysaccharide modulates astrocytic S100B secretion: a study in cerebrospinal fluid and astrocyte cultures from rats
Autor Guerra, Maria Cristina Azambuja Barea da Silveira
Tortorelli, Lucas Silva
Galland, Fabiana Andrea Barrera
Da Ré, Carollina Fraga
Bouchacourt, Elisa Negri
Engelke, Douglas Senna
Rodrigues, Letícia
Leite, Marina Concli
Goncalves, Carlos Alberto Saraiva
Abstract Background: Inflammatory responses in brain are primarily mediated by microglia, but growing evidence suggests a crucial importance of astrocytes. S100B, a calcium-binding protein secreted by astrocytes, has properties of a neurotrophic or an inflammatory cytokine. However, it is not known whether primary signals occurring during induction of an inflammatory response (e.g. lipopolysaccharide, LPS) directly modulate S100B. Methods: In this work, we evaluated whether S100B levels in cerebrospinal fluid (CSF) and serum of Wistar rats are affected by LPS administered by intraperitoneal (IP) or intracerebroventricular (ICV) injection, as well as whether primary astrocyte cultures respond directly to lipopolysaccharide. Results: Our data suggest that S100B secretion in brain tissue is stimulated rapidly and persistently (for at least 24 h) by ICV LPS administration. This increase in CSF S100B was transient when LPS was IP administered. In contrast to these S100B results, we observed an increase in in TNFa levels in serum, but not in CSF, after IP administration of LPS. In isolated astrocytes and in acute hippocampal slices, we observed a direct stimulation of S100B secretion by LPS at a concentration of 10 μg/mL. An involvement of TLR4 was confirmed by use of specific inhibitors. However, lower levels of LPS in astrocyte cultures were able to induce a decrease in S100B secretion after 24 h, without significant change in intracellular content of S100B. In addition, after 24 h exposure to LPS, we observed a decrease in astrocytic glutathione and an increase in astrocytic glial fibrillary acidic protein. Conclusions: Together, these data contribute to the understanding of the effects of LPS on astrocytes, particularly on S100B secretion, and help us to interpret cerebrospinal fluid and serum changes for this protein in neuroinflammatory diseases. Moreover, non-brain S100B-expressing tissues may be differentially regulated, since LPS administration did not lead to increased serum levels of S100B.
Contido em Journal of neuroinflammation. London. Vol. 8, (Oct. 2011), artigo 128, 11 p.
Assunto Astrócitos
Glutationa
Lipopolissacarídeos
Líquido cefalorraquidiano
Proteína glial fibrilar ácida
Proteínas S100
[en] Astrocyte, GFAP
[en] GFAP
[en] Glutathione
[en] LPS
[en] S100B
[en] TLR4
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/109952
Arquivos Descrição Formato
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