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Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets

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Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets

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Título Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
Autor Morais, Guilherme Loss de
Waterhouse, Peter M.
Margis, Rogerio
Abstract tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on deep sequencing data, was also analyzed under abiotic and biotic stresses. The results obtained here represent a useful starting point for future studies on tRFs in plants.
Contido em Biology direct. London. Vol. 8, no. 6 (17 May 2013), 5 p.
Assunto Arabidopsis thaliana
[en] Argonaute and Arabidopsis
[en] Small RNA
[en] tRFs
[en] tRNA-derived RNA fragments
[en] tRNAs
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/110062
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