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dc.contributor.authorThompson, Claudia Elizabethpt_BR
dc.contributor.authorSilva, Walter Orlando Beys dapt_BR
dc.contributor.authorSanti, Lucéliapt_BR
dc.contributor.authorOliveira, Markus Bergerpt_BR
dc.contributor.authorVainstein, Marilene Henningpt_BR
dc.contributor.authorGuimaraes, Jorge Almeidapt_BR
dc.contributor.authorVasconcelos, Ana Tereza Ribeiro dept_BR
dc.date.accessioned2015-02-14T02:19:48Zpt_BR
dc.date.issued2013pt_BR
dc.identifier.issn2191-0855pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/110156pt_BR
dc.description.abstractThe mangroves are among the most productive and biologically important environments. The possible presence of cellulolytic enzymes and microorganisms useful for biomass degradation as well as taxonomic and functional aspects of two Brazilian mangroves were evaluated using cultivation and metagenomic approaches. From a total of 296 microorganisms with visual differences in colony morphology and growth (including bacteria, yeast and filamentous fungus), 179 (60.5%) and 117 (39.5%) were isolated from the Rio de Janeiro (RJ) and Bahia (BA) samples, respectively. RJ metagenome showed the higher number of microbial isolates, which is consistent with its most conserved state and higher diversity. The metagenomic sequencing data showed similar predominant bacterial phyla in the BA and RJ mangroves with an abundance of Proteobacteria (57.8% and 44.6%), Firmicutes (11% and 12.3%) and Actinobacteria (8.4% and 7.5%). A higher number of enzymes involved in the degradation of polycyclic aromatic compounds were found in the BA mangrove. Specific sequences involved in the cellulolytic degradation, belonging to cellulases, hemicellulases, carbohydrate binding domains, dockerins and cohesins were identified, and it was possible to isolate cultivable fungi and bacteria related to biomass decomposition and with potential applications for the production of biofuels. These results showed that the mangroves possess all fundamental molecular tools required for building the cellulosome, which is required for the efficient degradation of cellulose material and sugar release.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofAMB Express. Heidelberg, GW. Vol. 3, no. 1 (2013), p. 65-83pt_BR
dc.rightsOpen Accessen
dc.subjectBrazilian mangroveen
dc.subjectMangues : Brasilpt_BR
dc.subjectCellulaseen
dc.subjectCelulasept_BR
dc.subjectBiomassapt_BR
dc.subjectMetagenomicsen
dc.subjectBiomass decompositionen
dc.subjectBacterial diversityen
dc.titleA potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of brazilian mangrovespt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000931565pt_BR
dc.type.originEstrangeiropt_BR


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