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Regulation of lung oxidative damage by endogenous superoxide dismutase in sepsis

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Regulation of lung oxidative damage by endogenous superoxide dismutase in sepsis

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Título Regulation of lung oxidative damage by endogenous superoxide dismutase in sepsis
Autor Constantino, Larissa de Souza
Gonçalves, Renata Casagrande
Giombelli, Vinicius Renne
Tomasi, Cristiane Damiani
Vuolo, Franciele Silva
Kist, Luiza Wilges
Oliveira, Giovanna Medeiros Tavares de
Pasquali, Matheus Augusto de Bittencourt
Bogo, Mauricio Reis
Mauad, Thais
Horn Junior, Adolfo
Melo, Karen Vieira
Fernandes, Christiane
Moreira, Jose Claudio Fonseca
Ritter, Cristiane
Dal Pizzol, Felipe
Abstract Background: The purpose of this research is to study the relationship between superoxide dismutase (SOD) and lung redox state in an animal model of sepsis. Methods: Sepsis was induced in rats by the cecal ligation and perforation model (CLP). After 3, 6, and 12 h, CLP protein content and expression of SOD1, SOD2, and SOD3 were evaluated, and SOD activity was assessed. Oxidative damage was determined by quantifying nitrotyrosine content. Lung localization of SOD3 was performed by immunohistochemistry. The protective effect of a SOD mimetic on oxidative damage, inflammation, and lung permeability was assessed 12 and 24 h after sepsis induction. Results: Lung levels of SOD1 decreased 3 and 12 h after sepsis, but SOD2 and SOD3 increased, as well as SOD activity. These alterations were not associated with changes in sod gene expression. Nitrotyrosine levels increased 3 and 12 h after sepsis. The administration of a SOD mimetic decreased nitrotyrosine and proinflammatory cytokine levels and improved lung permeability. Conclusions: SOD2 and SOD3 increased after sepsis induction, but this was insufficient to protect the lung. Treatments based on SOD mimetics could have a role in lung injury associated with sepsis.
Contido em Intensive care medicine experimental. [S. l. : Springer]. Vol. 2 (23 May 2014), p. 17 [11 p.]
Assunto Oxirredução
Pulmão
Sepse
Superóxido dismutase
[en] Lung
[en] NO
[en] Redox state
[en] Sepsis
[en] SOD
[en] SOD mimetic
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/110221
Arquivos Descrição Formato
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