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Messenger RNA levels of podocyte-associated proteins in subjects with different degrees of glucose tolerance with or without nephropathy

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Messenger RNA levels of podocyte-associated proteins in subjects with different degrees of glucose tolerance with or without nephropathy

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Título Messenger RNA levels of podocyte-associated proteins in subjects with different degrees of glucose tolerance with or without nephropathy
Autor Nascimento, Jonathan Fraportti do
Canani, Luis Henrique Santos
Gerchman, Fernando
Rodrigues, Patrícia Garcia
Joelsons, Gabriel
Santos, Mariane dos
Pereira, Sane Vianna
Veronese, Francisco José Veríssimo
Abstract Background: To investigate gene expression of podocyte-specific proteins in urine of diabetes and prediabetes subjects and the association of these proteins with albuminuria. Methods: Fifteen controls, 19 prediabetes, and 67 diabetes subjects were included. Messenger RNA of nephrin, podocin, podocalyxin, synaptopodin, TRPC6, alpha-actinin-4, and TGF-β1 were measured using RT-PCR. Podocyte marker expression was correlated with albuminuria, glycemic control, and renal function. The diagnostic performance of the genes used to detect increased albuminuria was assessed using ROC curves and Poisson regressions. Results: Podocyte marker expression was significantly higher in diabetic subjects. Urinary nephrin was correlated with increasing levels of albuminuria; risk of albuminuria increased by 20% for every one-unit increase in the log10 of nephrin mRNA. Nephrinuria was found in 53%, 71%, and 90% of normo-, micro-, and macroalbuminuric diabetes subjects, respectively (p = 0.023). Urinary nephrin, podocalyxin, TRPC6, podocin, and alpha actinin-4 were correlated with glycemic control and albuminuria but not with renal function. Conclusions: Diabetes subjects had higher urinary mRNA levels of podocyte proteins than nondiabetic subjects, even the normoalbuminuric patients. Nephrinuria was correlated with diabetic nephrophathy stage and predicted pathological albuminuria. Urinary mRNA levels of podocyte markers of prediabetic subjects did not differ from controls.
Contido em BMC nephrology. London. Vol. 2013 (Oct. 2013), 10p.
Assunto Estado pré-diabético
Nefropatias diabéticas
Podócitos
[en] Diabetic nephropathy
[en] Nephrin
[en] Podocyte
[en] Prediabetes
[en] TRPC6
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/111810
Arquivos Descrição Formato
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