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Comparative genome analysis of entomopathogenic fungi reveals a complex set of secreted proteins

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Comparative genome analysis of entomopathogenic fungi reveals a complex set of secreted proteins

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Título Comparative genome analysis of entomopathogenic fungi reveals a complex set of secreted proteins
Autor Staats, Charley Christian
Junges, Angela
Guedes, Rafael Lucas Muniz
Thompson, Claudia Elizabeth
Morais, Guilherme Loss de
Boldo, Juliano Tomazzoni
Almeida, Luiz Gonzaga Paula de
Andreis, Fábio Carrer
Gerber, Alexandra Lehmkuhl
Oliveira, Nicolau Sbaraini
Paixão, Rana Louise de Andrade da
Broetto, Leonardo
Landell, Melissa Fontes
Santi, Lucélia
Silva, Walter Orlando Beys da
Silveira, Carolina Pereira
Serrano, Thaiane Rispoli
Oliveira, Eder Silva de
Silva, Lívia Kmetzsch Rosa e
Vainstein, Marilene Henning
Vasconcelos, Ana Tereza Ribeiro de
Schrank, Augusto
Abstract Background: Metarhizium anisopliae is an entomopathogenic fungus used in the biological control of some agricultural insect pests, and efforts are underway to use this fungus in the control of insect-borne human diseases. A large repertoire of proteins must be secreted by M. anisopliae to cope with the various available nutrients as this fungus switches through different lifestyles, i.e., from a saprophytic, to an infectious, to a plant endophytic stage. To further evaluate the predicted secretome of M. anisopliae, we employed genomic and transcriptomic analyses, coupled with phylogenomic analysis, focusing on the identification and characterization of secreted proteins. Results: We determined the M. anisopliae E6 genome sequence and compared this sequence to other entomopathogenic fungi genomes. A robust pipeline was generated to evaluate the predicted secretomes of M. anisopliae and 15 other filamentous fungi, leading to the identification of a core of secreted proteins. Transcriptomic analysis using the tick Rhipicephalus microplus cuticle as an infection model during two periods of infection (48 and 144 h) allowed the identification of several differentially expressed genes. This analysis concluded that a large proportion of the predicted secretome coding genes contained altered transcript levels in the conditions analyzed in this study. In addition, some specific secreted proteins from Metarhizium have an evolutionary history similar to orthologs found in Beauveria/Cordyceps. This similarity suggests that a set of secreted proteins has evolved to participate in entomopathogenicity. Conclusions: The data presented represents an important step to the characterization of the role of secreted proteins in the virulence and pathogenicity of M. anisopliae.
Contido em BMC Genomics. London. Vol. 15, no. 822 (Sep. 2014), p. 1-18
Assunto Fungo entomopatogenico
Genoma
[en] Entomopathogenic fungi
[en] Genome sequence
[en] Phylogenomics
[en] Secretome
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/115041
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