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Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection

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Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection

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Título Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection
Autor Bencke, Marta
Cabreira, Caroline
Strohm, Beatriz Wiebke
Bücker Neto, Lauro
Mancini, Estefania
Osorio, Marina Borges
Homrich, Milena Schenkel
Zolet, Andreia Carina Turchetto
Carvalho, Mayra C.C.G. de
Stolf, Renata
Weber, Ricardo Luís Mayer
Westergaard, Gastón
Castagnaro, Atilio P.
Abdelnoor, Ricardo Vilela
Guimarães, Francismar Correa Marcelino
Margis-Pinheiro, Márcia
Bodanese-Zanettini, Maria Helena
Abstract Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified. Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants. Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.
Contido em BMC Plant biology. London. Vol. 14, no. 236, (Sep. 2014), p. 1-18
Assunto Fungos
Glycine max
Soja
[en] Asian Soybean Rust
[en] Functional analysis
[en] Fungus resistance
[en] Genetic transformation
[en] Glycine max
[en] Transcription factors
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/115078
Arquivos Descrição Formato
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