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Saliva, supragingival biofilm and root canals can harbor gene associated with resistance to lactamic agents

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Saliva, supragingival biofilm and root canals can harbor gene associated with resistance to lactamic agents

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Título Saliva, supragingival biofilm and root canals can harbor gene associated with resistance to lactamic agents
Autor Moraes, Ludmila Coutinho
Parolo, Clarissa Cavalcanti Fatturi
Ferreira, Maria Beatriz Cardoso
Só, Marcus Vinicius Reis
Montagner, Francisco
Abstract This study aimed to determine the presence of Prevotella strains and genes associated with resistance to lactamics in different oral niches from patients with/without primary endodontic infections. Saliva (S) and supragingival biofilm (SB) were collected from three patient groups: Group I – no endodontic infection (n = 15); Group II – acute endodontic infection (n = 12); and Group III – chronic endodontic infection (n = 15). Root canal (RC) samples were collected from Groups II and III. The presence of P. intermedia, P nigrescens, P. tannerae and cfxA/cfxA2 gene was assessed by PCR. The cfxA/cfxA2 gene was not detected in all environments within the same patient. The cfxA/cfxA2 gene was present in 23.81% of S samples, 28.57% of SB samples, and 7.41% of RC samples. Prevotella species were detected in 53.97%, 47.62% and 34.56% of the S, SB, and RC samples, respectively. P. intermedia had a high frequency in saliva samples from Group 3. Saliva samples from Group 1 had higher detection rates of P. nigrescens than did Groups 2 and 3. Patients without endodontic disease had high frequencies of P. nigrescens in the SB samples. The presence or absence of spontaneous symptoms was not related to the detection rates for resistance genes in the RC samples. Saliva, supragingival biofilm and root canals can harbor resistant bacteria. The presence of symptomatology did not increase the presence of the cfxA/cfxA2 gene in the supragingival biofilm and inside root canals.
Contido em Brazilian oral research. São Paulo. Vol. 29, no. 1 (2015), p. 1-6
Assunto Biologia molecular
Endodontia
Genes
Resistência a medicamentos
[en] Drug resistance
[en] Endodontics
[en] Molecular biology
[en] Mouth
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/115846
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