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Intrinsic terminators in Mycoplasma hyopneumoniae transcription

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Intrinsic terminators in Mycoplasma hyopneumoniae transcription

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Título Intrinsic terminators in Mycoplasma hyopneumoniae transcription
Autor Fritsch, Tiago Ebert
Siqueira, Franciele Maboni
Schrank, Irene Silveira
Abstract Background: Mycoplasma hyopneumoniae, an important pathogen of swine, exhibits a low guanine and cytosine (GC) content genome. M. hyopneumoniae genome is organised in long transcriptional units and promoter sequences have been mapped upstream of all transcription units. These analysis provided insights into the gene organisation and transcription initiation at the genome scale. However, the presence of transcriptional terminator sequences in the M. hyopneumoniae genome is poorly understood. Results: In silico analyses demonstrated the presence of putative terminators in 82% of the 33 monocistronic units (mCs) and in 74% of the 116 polycistronic units (pCs) considering different classes of terminators. The functional activity of 23 intrinsic terminators was confirmed by RT-PCR and qPCR. Analysis of all terminators found by three software algorithms, combined with experimental results, allowed us to propose a pattern of RNA hairpin formation during the termination process and to predict the location of terminators in the M. hyopneumoniae genome sequence. Conclusions: The stem-loop structures of intrinsic terminators of mycoplasma diverge from the pattern of terminators found in other bacteria due the low content of guanine and cytosine. In M. hyopneumoniae, transcription can end after a transcriptional unit and before its terminator sequence and can also continue past the terminator sequence with RNA polymerases gradually releasing the RNA.
Contido em BMC Genomics. London. Vol. 16, no. 273 (2015), p. 1-12
Assunto Mycoplasma hyopneumoniae
Transcrição genética
[en] Intrinsic terminator
[en] Transcriptional units
[en] Transcription termination
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/122623
Arquivos Descrição Formato
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