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Podocyte-associated mRNA profiles in kidney tissue and in urine of patients with active lupus nephritis

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Podocyte-associated mRNA profiles in kidney tissue and in urine of patients with active lupus nephritis

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Título Podocyte-associated mRNA profiles in kidney tissue and in urine of patients with active lupus nephritis
Autor Santos, Mariane dos
Bringhenti, Rafael Nazario
Rodrigues, Patrícia Garcia
Nascimento, Jonathan Fraportti do
Pereira, Sane Vianna
Zancan, Rafael
Monticielo, Odirlei André
Gasparin, Andrese Aline
Castro, Waldir Pedro de
Veronese, Francisco José Veríssimo
Abstract Aim: Glomerular deposition of immune complexes and inflammation induce podocyte injury in lupus nephritis (LN). This study hypothesized that the severity of the histological lesions of LN affects podocyte-associated mRNAs profiles in kidney tissue and in urine. Methods: Thirty-three patients with LN were grouped according to the presence of mild mesangial (classes I and II) or moderate-to-severe immune complex deposition, proliferation and/or inflammation (classes III, IV and V) in kidney biopsy. Tissue and urine mRNA of nephrin, podocin, podocalyxin, α-actinin-4, transient receptor potential cation channel 6, and of growth factors VEGF-A and TGF-β1 and the transcription factor FOXP3 were measured using real time polymerase chain reaction. These mRNAs were correlated with histological severity of LN, extent of glomerular immune deposits, and tissue infiltrating cells. Results: Podocyte-associated mRNAs were inhibited in renal tissue of patients with LN irrespective of histological class when compared to controls. However, significantly higher expression of podocyte mRNAs in urine, including those of growth factors and FOXP3, were found in patients with moderate-to-severe nephritis, mostly in class III and IV proliferative forms. The number of invading CD8+ T cells, B cells and macrophages correlated positively with urine podocyte-associated mRNAs. Urine podocyte mRNAs also correlated with proteinuria. Conclusions: Inhibition of podocyte-associated mRNAs in kidney tissue suggests that podocyte injury occurs regardless of class severity of LN. Increased urinary excretion of podocyte mRNAs, mostly in patients with moderate-to-severe lesions, may reflect a greater burden of glomerular damage with detachment of podocytes into the urine.
Contido em International journal of clinical and experimental pathology. Madison. Vol. 8, no. 5 (2015), p. 4600-4613
Assunto Fator A de crescimento do endotélio vascular
Lupus eritematoso sistêmico
Nefrite lúpica
Podócitos
[en] FOXP3
[en] Lupus nephritis
[en] Podocin
[en] Podocytes
[en] Systemic lupus erythematosus
[en] Vascular endothelial growth factor
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/129900
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