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Genomic instability in human lymphocytes from male users of crack cocaine

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Genomic instability in human lymphocytes from male users of crack cocaine

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Título Genomic instability in human lymphocytes from male users of crack cocaine
Autor Freitas, Thiago Aley Brites de
Palazzo, Roberta Passos
Andrade, Fabiana Michelsen de
Reichert, César Luis
Pechansky, Flavio
Kessler, Felix Henrique Paim
Farias, Caroline Brunetto de
Andrade, Gisele Gomes de
Leistner-Segal, Sandra
Maluf, Sharbel Weidner
Abstract Recent research suggests that crack cocaine use alters systemic biochemical markers, like oxidative damage and inflammation markers, but very few studies have assessed the potential effects of crack cocaine at the cellular level. We assessed genome instability by means of the comet assay and the cytokinesis-block micronucleus technique in crack cocaine users at the time of admission to a rehabilitation clinic and at two times after the beginning of withdrawal. Thirty one active users of crack cocaine and forty control subjects were evaluated. Comparison between controls and crack cocaine users at the first analysis showed significant differences in the rates of DNA damage (p = 0.037). The frequency of micronuclei (MN) (p < 0.001) and nuclear buds (NBUDs) (p < 0.001) was increased, but not the frequency of nucleoplasmic bridges (NPBs) (p = 0.089). DNA damage decreased only after the end of treatment (p < 0.001). Micronuclei frequency did not decrease after treatment, andnuclear buds increased substantially. The results of this study reveal the genotoxic and mutagenic effects of crack cocaine use in human lymphocytes and pave the way for further research on cellular responses and the possible consequences of DNA damage, such as induction of irreversible neurological disease and cancer.
Contido em International journal of environmental research and public health. Basel. Vol. 11, no. 10 (Sept. 26, 2014), p. 10003-10015
Assunto Cocaína crack
Instabilidade genômica
Linfócitos
Masculino
Usuários de drogas
[en] Comet assay
[en] Crack cocaine
[en] DNA damage
[en] Drug withdrawal
[en] Micronucleus
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/129909
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