Repositório Digital

A- A A+

Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals?

.

Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals?

Mostrar registro completo

Estatísticas

Título Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals?
Autor Melo, Tiago André Fontoura de
Gründling, Grasiela Sabrina Longhi
Montagner, Francisco
Scarparo, Roberta Kochenborger
Figueiredo, Jose Antonio Poli de
Vier-Pelisser, Fabiana Vieira
Abstract The present study aimed to determine the feasibility of using bovine teeth as a suitable alternative for human teeth, in experiments involving in vitro endotoxin contamination. Twenty bovine central incisors and 20 human single-root premolars had their dental crowns removed and root lengths set at 16 mm. Root canals were prepared up to #60 K-file size and sterilized with cobalt-60 gamma irradiation (20 kGy, 6 h). The teeth were randomly divided into four groups: G1-bovine teeth (bovine negative control, n = 10), G2-human teeth (human negative control, n = 10), G3-bovine teeth, inoculated with Escherichia coli (055:B55) LPS, and G4-human teeth inoculated with E. coli LPS. The G1 and G2 groups were exposed to apyrogenic water. After the teeth had been incubated at 37 °C and atmospheric humidity for 24 h, the samples of solutions in the main canals were collected with apyrogenic absorbent paper tips. LPS levels were quantified using Limulus Amebocyte Lysate assay. The data obtained were statistically analyzed using one-way ANOVA, with a significance level of 5%. A high amount of endotoxin was detected in the inoculated human teeth (G4) when compared to the sterilized teeth (G2), as well as in the inoculated bovine teeth (G3) when compared to the inoculated human teeth (G4). However, there was no statistical difference between bovine teeth before and after the E. coli endotoxin inoculation. Therefore, under the mentioned experimental conditions, the use of bovine teeth should not be a choice for laboratory research on endotoxin contamination.
Contido em Brazilian oral research. São Paulo. Vol. 29, n. 1 (2015), p. 1-6
Assunto Canais radiculares
Endotoxinas
Polpa dentaria
[en] Cattle
[en] Decontamination
[en] Dental Pulp Cavity
[en] Endotoxins
[en] Humans
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/131772
Arquivos Descrição Formato
000977908.pdf (258.3Kb) Texto completo (inglês) Adobe PDF Visualizar/abrir

Este item está licenciado na Creative Commons License

Este item aparece na(s) seguinte(s) coleção(ões)


Mostrar registro completo

Percorrer



  • O autor é titular dos direitos autorais dos documentos disponíveis neste repositório e é vedada, nos termos da lei, a comercialização de qualquer espécie sem sua autorização prévia.
    Projeto gráfico elaborado pelo Caixola - Clube de Criação Fabico/UFRGS Powered by DSpace software, Version 1.8.1.