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Liver δ-aminolevulinate dehydratase activity is inhibited by neonicotinoids and restored by antioxidant agents

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Liver δ-aminolevulinate dehydratase activity is inhibited by neonicotinoids and restored by antioxidant agents

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Título Liver δ-aminolevulinate dehydratase activity is inhibited by neonicotinoids and restored by antioxidant agents
Autor Sauer, Elisa
Moro, Angela Maria
Brucker, Natália
Nascimento, Sabrina Nunes do
Gauer, Bruna
Fracasso, Rafael
Gioda, Adriana
Beck, Ruy Carlos Ruver
Moreira, Jose Claudio Fonseca
Eifler-Lima, Vera Lucia
Garcia, Solange Cristina
Abstract Neonicotinoids represent the most used class of insecticides worldwide, and their precursor, imidacloprid, is the most widely marketed. The aim of this study was to evaluate the effect of imidacloprid on the activity of hepatic δ-aminolevulinate dehydratase (δ-ALA-D), protective effect of potential antioxidants against this potential effect and presence of chemical elements in the constitution of this pesticide. We observed that δ-ALA-D activity was significantly inhibited by imidacloprid at all concentrations tested in a dose-dependent manner. The IC50 value was obtained and used to evaluate the restoration of the enzymatic activity. δ-ALA-D inhibition was completely restored by addition of dithiotreitol (DTT) and partly by ZnCl2, demonstrating that the inhibition occurs by oxidation of thiol groups and by displacement of the Zn (II), which can be explained by the presence of chemical elements found in the constitution of pesticides. Reduced glutathione (GSH) had the best antioxidant effect against to δ-ALA-D inhibition caused by imidacloprid, followed by curcumin and resveratrol. It is well known that inhibition of the enzyme δ-ALA-D may result in accumulation of its neurotoxic substrate (δ-ALA), in this line, our results suggest that further studies are needed to investigate the possible neurotoxicity induced by neonicotinoids and the involvement of antioxidants in cases of poisoning by neonicotinoids.
Contido em International Journal of Environmental Research and Public Health. Basel. Vol. 11, no.11 (2014), p. 11676-11690
Assunto Inseticidas
Praguicidas
Toxicologia
[en] Antioxidants
[en] Neonicotinoids
[en] Toxicity
[en] δ-ALA-D activity
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/140172
Arquivos Descrição Formato
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