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Effects of two types of melatonin-loaded nanocapsules with distinct supramolecular structures : Polymeric (NC) and Lipid-Core Nanocapsules (LNC) on bovine embryo culture model

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Effects of two types of melatonin-loaded nanocapsules with distinct supramolecular structures : Polymeric (NC) and Lipid-Core Nanocapsules (LNC) on bovine embryo culture model

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Título Effects of two types of melatonin-loaded nanocapsules with distinct supramolecular structures : Polymeric (NC) and Lipid-Core Nanocapsules (LNC) on bovine embryo culture model
Autor Komninou, Eliza Rossi
Remião, Mariana Härter
Lucas, Caroline Gomes
Domingues, William Broges
Basso, Andréa Cristina
Jornada, Denise Soledade
Deschamps, João Carlos
Beck, Ruy Carlos Ruver
Pohlmann, Adriana Raffin
Bordignon, Vilceu
Seixas, Fabiana Kömmling
Campos, Vinicius Farias
Guterres, Silvia Stanisçuaski
Collares, Tiago Veiras
Abstract Melatonin has been used as a supplement in culture medium to improve the efficiency of in vitro produced mammalian embryos. Through its ability to scavenge toxic oxygen derivatives and regulate cellular mRNA levels for antioxidant enzymes, this molecule has been shown to play a protective role against damage by free radicals, to which in vitro cultured embryos are exposed during early development. In vivo and in vitro studies have been performed showing that the use of nanocapsules as active substances carriers increases stability, bioavailability and biodistribution of drugs, such as melatonin, to the cells and tissues, improving their antioxidant properties. These properties can be modulated through the manipulation of formula composition, especially in relation to the supramolecular structures of the nanocapsule core and the surface area that greatly influences drug release mechanisms in biological environments. This study aimed to evaluate the effects of two types of melatonin-loaded nanocapsules with distinct supramolecular structures, polymeric (NC) and lipid-core (LNC) nanocapsules, on in vitro cultured bovine embryos. Embryonic development, apoptosis, reactive oxygen species (ROS) production, and mRNA levels of genes involved in cell apoptosis, ROS and cell pluripotency were evaluated after supplementation of culture medium with non-encapsulated melatonin (Mel), melatonin-loaded polymeric nanocapsules (Mel-NC) and melatonin-loaded lipid-core nanocapsules (Mel-LNC) at 10−6, 10−9, and 10−12 M drug concentrations. The highest hatching rate was observed in embryos treated with 10−9 M Mel-LNC. When compared to Mel and Mel-NC treatments at the same concentration (10−9 M), Mel-LNC increased embryo cell number, decreased cell apoptosis and ROS levels, down-regulated mRNA levels of BAX, CASP3, and SHC1 genes, and upregulated mRNA levels of CAT and SOD2 genes. These findings indicate that nanoencapsulation with LNC increases the protective effects of melatonin against oxidative stress and cell apoptosis during in vitro embryo culture in bovine species.
Contido em PLoS ONE. San Francisco. Vol. 11, no. 6 (June 2016), e0157561, p. 1-19
Assunto Antioxidantes
Estresse oxidativo
Melatonina
Nanocapsulas
Nanocapsulas poliméricas
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/143825
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