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Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis

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Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis

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Título Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis
Autor Barros, Muriel Primon de
Rigo, Graziela de Vargas
Frasson, Amanda Piccoli
Santos, Odelta dos
Smiderle, Lisiane
Almeida, Silvana Maria de
Macedo, Alexandre José
Tasca, Tiana
Abstract Trichomonas vaginalis is a flagellate protozoan that parasitises the urogenital human tract and causes trichomoniasis. During the infection, the acquisition of nutrients, such as iron and purine and pyrimidine nucleosides, is essential for the survival of the parasite. The enzymes for purinergic signalling, including adenosine deaminase (ADA), which degrades adenosine to inosine, have been characterised in T. vaginalis. In the evaluation of the ADA profile in different T. vaginalis isolates treated with different iron sources or with limited iron availability, a decrease in activity and an increase in ADA gene expression after iron limitation by 2,2-bipyridyl and ferrozine chelators were observed. This supported the hypothesis that iron can modulate the activity of the enzymes involved in purinergic signalling. Under bovine serum limitation conditions, no significant differences were observed. The results obtained in this study allow for the assessment of important aspects of ADA and contribute to a better understanding of the purinergic system in T. vaginalis and the role of iron in establishing infection and parasite survival.
Contido em Memórias do Instituto Oswaldo Cruz. Vol. 110, n. 7 (2015), p. 877-883
Assunto Farmácia
[en] Adenosine deaminase
[en] Iron chelators
[en] Purinergic system
[en] Trichomonas vaginalis
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/147007
Arquivos Descrição Formato
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