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dc.contributor.authorMelo, Tiago André Fontoura dept_BR
dc.contributor.authorGründling, Grasiela Sabrina Longhipt_BR
dc.contributor.authorMontagner, Franciscopt_BR
dc.contributor.authorScur, Alcionept_BR
dc.contributor.authorSteier, Liviupt_BR
dc.contributor.authorScarparo, Roberta Kochenborgerpt_BR
dc.contributor.authorFigueiredo, Jose Antonio Poli dept_BR
dc.contributor.authorVier-Pelisser, Fabiana Vieirapt_BR
dc.date.accessioned2016-08-11T02:15:28Zpt_BR
dc.date.issued2016pt_BR
dc.identifier.issn1806-8324pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/147051pt_BR
dc.description.abstractThe present study aims to verify the effect of ozone gas (OZY® System) and high frequency electric pulse (Endox® System) systems on human root canals previously contaminated with Escherichia coli lipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths standardized to 16 mm. The root canals were prepared up to #60 hand K-files and sterilized using gamma radiation with cobalt 60. The specimens were divided into the following five groups (n = 10) based on the disinfection protocol used: OZY® System, one 120-second-pulse (OZY 1p); OZY® System, four 24-second-pulses (OZY 4p); and Endox® System (ENDOX). Contaminated and non-contaminated canals were exposed only to apyrogenic water and used as positive (C+) and negative (C-) controls, respectively. LPS (O55:B55) was administered in all root canals except those belonging to group C-. After performing disinfection, LPS samples were collected from the canals using apyrogenic paper tips. Limulus Amoebocyte Lysate (LAL) was used to quantify the LPS levels, and the data obtained was analyzed using one-way ANOVA. The disinfection protocols used were unable to reduce the LPS levels significantly (p = 0.019). The use of ozone gas and high frequency electric pulses was not effective in eliminating LPS from the root canals.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoporpt_BR
dc.relation.ispartofBrazilian oral research. São Paulo. Vol. 30, no. 1 (2016), p. e 19, 1-6pt_BR
dc.rightsOpen Accessen
dc.subjectEndodontiapt_BR
dc.subjectEndodonticsen
dc.subjectEndotoxinsen
dc.subjectMicrobiologiapt_BR
dc.subjectMicrobiologyen
dc.subjectMateriais odontológicospt_BR
dc.subjectCanais radiculares : Tratamentopt_BR
dc.subjectRoot canal therapyen
dc.titleLPS levels in root canals after the use of ozone gas and high frequency electrical pulsespt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000988579pt_BR
dc.type.originNacionalpt_BR


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