Cranial vault reconstruction with bone morphogenetic protein, calcium phosphate, acellular dermal matrix, and calcium alginate in mice
dc.contributor.author | Portinho, Ciro Paz | pt_BR |
dc.contributor.author | Santos, Luis Alberto dos | pt_BR |
dc.contributor.author | Cerski, Carlos Thadeu Schmidt | pt_BR |
dc.contributor.author | Rivero, Raquel Camara | pt_BR |
dc.contributor.author | Collares, Marcus Vinicius Martins | pt_BR |
dc.date.accessioned | 2016-08-13T02:15:54Z | pt_BR |
dc.date.issued | 2014 | pt_BR |
dc.identifier.issn | 0102-8650 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/147197 | pt_BR |
dc.description.abstract | ABSTRACTPURPOSE: To evaluate experimental cranial vault reconstructions, by combining bone morphogenetic protein type 2 (BMP-2) and different matrices.METHODS: Fourty-nine animals were initially included (seven per group). We designed an experimental, open, prospective and comparative study, divided in seven groups: 1 – BMP-2+calcium phosphate (BT); 2 – BMP-2+acellular dermal matrix (BM); 3 – BMP-2+calcium alginate (BA); 4 – TCP; 5 – MDM; 6 – ALG; 7 – Bone autograft (BAG). A bone failure was created in left parietal bone of adult male mice. At the same procedure reconstruction was performed. After five weeks, animals were sacrificed, and reconstruction area was removed to histological analysis. After exclusion due to death or infection, thirty-eight animals were evaluated (BT=5; BM=6; BA=6; TCP=7; MDM=3; ALG=6; BAG=5).RESULTS: A higher incidence of infection has occurred in MDM group (57%, P=0.037). In cortical fusion, groups BAG, TCP, and BMP-2+TCP (BT) obtained the best scores, comparing to the others (P=0.00846). In new bone formation, groups BT, BAG, and TCP have presented the best scores (P=0.00835). When neovascularization was considered, best groups were BMP-2+MDM (BM), BMP-2+ALG (BA), TCP, and MDM (P=0.001695). BAG group was the best in bone marrow formation, followed by groups BT and TCP (P=0.008317). CONCLUSIONS: Bone morphogenetic protein type 2 increased bone regeneration in experimental skull reconstruction, especially when combined to calcium phosphate. Such association was even comparable to bone autograft, the gold-standard treatment, in some histological criteria. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Acta cirúrgica brasileira. São Paulo, SP. Vol. 29, no. 10 (Oct. 2014), p. 622-632 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Bone and bones | en |
dc.subject | Osso e ossos | pt_BR |
dc.subject | Bone transplantation | en |
dc.subject | Engenharia de tecidos | pt_BR |
dc.subject | Tissue engineering | en |
dc.subject | Bone matrix | en |
dc.subject | Craniofacial abnormalities | en |
dc.subject | Mice | en |
dc.title | Cranial vault reconstruction with bone morphogenetic protein, calcium phosphate, acellular dermal matrix, and calcium alginate in mice | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 000990792 | pt_BR |
dc.type.origin | Nacional | pt_BR |
Este item está licenciado na Creative Commons License
-
Artigos de Periódicos (39096)Engenharias (2395)
-
Artigos de Periódicos (39096)Ciências da Saúde (10544)