Repositório Digital

A- A A+

Feline immunodeficiency virus Vif N-Terminal residues selectively counteract feline APOBEC3s

.

Feline immunodeficiency virus Vif N-Terminal residues selectively counteract feline APOBEC3s

Mostrar registro completo

Estatísticas

Título Feline immunodeficiency virus Vif N-Terminal residues selectively counteract feline APOBEC3s
Autor Gu, Qinyong
Zhang, Zeli
Ortiz, Lucía Cano
Franco, Ana Claudia
Häussinger, Dieter
Münk, Carsten
Abstract Feline immunodeficiency virus (FIV) Vif protein counteracts feline APOBEC3s (FcaA3s) restriction factors by inducing their proteasomal degradation. The functional domains in FIV Vif for interaction with FcaA3s are poorly understood. Here, we have identified several motifs in FIV Vif that are important for selective degradation of different FcaA3s. Cats (Felis catus) express three types of A3s: single-domain A3Z2, single-domain A3Z3, and double-domain A3Z2Z3. We proposed that FIV Vif would selectively interact with the Z2 and the Z3 A3s. Indeed, we identified two N-terminal Vif motifs (12LF13 and 18GG19) that specifically interacted with the FcaA3Z2 protein but not with A3Z3. In contrast, the exclusive degradation of FcaA3Z3 was regulated by a region of three residues (M24, L25, and I27). Only a FIV Vif carrying a combination of mutations from both interaction sites lost the capacity to degrade and counteract FcaA3Z2Z3. However, alterations in the specific A3s interaction sites did not affect the cellular localization of the FIV Vif protein and binding to feline A3s. Pulldown experiments demonstrated that the A3 binding region localized to FIV Vif residues 50 to 80, outside the specific A3 interaction domain. Finally, we found that the Vif sites specific to individual A3s are conserved in several FIV lineages of domestic cat and nondomestic cats, while being absent in the FIV Vif of pumas. Our data support a complex model of multiple Vif-A3 interactions in which the specific region for selective A3 counteraction is discrete from a general A3 binding domain.
Contido em Journal of Virology. Washington, DC. Vol. 90, no. 23, (Dec. 2016), p. 10545-10557
Assunto Felinos
Vírus da imunodeficiência felina
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/165164
Arquivos Descrição Formato
001044425.pdf (2.209Mb) Texto completo Adobe PDF Visualizar/abrir

Este item está licenciado na Creative Commons License

Este item aparece na(s) seguinte(s) coleção(ões)


Mostrar registro completo

Percorrer



  • O autor é titular dos direitos autorais dos documentos disponíveis neste repositório e é vedada, nos termos da lei, a comercialização de qualquer espécie sem sua autorização prévia.
    Projeto gráfico elaborado pelo Caixola - Clube de Criação Fabico/UFRGS Powered by DSpace software, Version 1.8.1.