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Salt stress affects mRNA editing in soybean chloroplasts

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Salt stress affects mRNA editing in soybean chloroplasts

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Título Salt stress affects mRNA editing in soybean chloroplasts
Autor Rodrigues, Nureyev Ferreira
Fonseca, Guilherme Cordenonsi da
Kulcheski, Franceli Rodrigues
Margis, Rogerio
Abstract Soybean, a crop known by its economic and nutritional importance, has been the subject of several studies that assess the impact and the effective plant responses to abiotic stresses. Salt stress is one of the main environmental stresses and negatively impacts crop growth and yield. In this work, the RNA editing process in the chloroplast of soybean plants was evaluated in response to a salt stress. Bioinformatics approach using sRNA and mRNA libraries were employed to detect specific sites showing differences in editing efficiency. RT-qPCR was used to measure editing efficiency at selected sites. We observed that transcripts of NDHA, NDHB, RPS14 and RPS16 genes presented differences in coverage and editing rates between control and salt-treated libraries. RT-qPCR assays demonstrated an increase in editing efficiency of selected genes. The salt stress enhanced the RNA editing process in transcripts, indicating responses to components of the electron transfer chain, photosystem and translation complexes. These increases can be a response to keep the homeostasis of chloroplast protein functions in response to salt stress.
Contido em Genetics and molecular biology. Ribeirão Preto, SP. Vol. 40, no. 1, suppl. 1 (2017), p. 200-208
Assunto Cloroplasto
Estresse salino
RNA
[en] Chloroplast
[en] PPR
[en] RNA editing
[en] Salt stress
[en] Small RNA
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/166148
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