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Fluorescent test accurately predicts leak of ischemic colon anastomosis in rats

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Fluorescent test accurately predicts leak of ischemic colon anastomosis in rats

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Título Fluorescent test accurately predicts leak of ischemic colon anastomosis in rats
Autor Moschetti, Laura
Ghezzi, Tiago Leal
Müller, Beliza Gomes
Dias, Adriano Basso
Corleta, Oly Campos
Abstract Purpose: To investigate if fluorescein fluorescent test can predict dehiscence in a model of ischemic colonic anastomosis in rats. Methods: This experimental controlled trial randomly assigned 55 rats to four groups. Anastomoses were performed in non-ischemic colon segments (control group) and in ischemic colon segments measuring 1, 2 or 3 cm long (groups 1, 2 and 3, respectively). Fluorescein was injected and the tissues were examined under ultraviolet light. Seven days later, a second-look surgery was performed to check for the presence or absence of anastomosis dehiscence. Results: Twenty-four rats presented anastomotic dehiscence during the second-look surgery. Reticular and nonfluorescent patterns were significantly associated with the occurrence of anastomotic dehiscence. Fluorescein fluorescence had a sensitivity of 95.8%, specificity of 89.2%, positive predictive value of 88.4%, negative predictive value of 96.2%, and accuracy of 92.3% to predict anastomotic dehiscence. Conclusion: Fluorescein fluorescent test can accurately predict leak in a model of ischemic colonic anastomosis in rats.
Contido em Acta cirúrgica brasileira. São Paulo. Vol. 32, n. 6 (jun. 2017), p. 440-448
Assunto Anastomose cirúrgica
Fístula anastomótica
Fluorescência
Ratos
[en] Anastomosis
[en] Anastomotic Leak
[en] Blood Supply
[en] Fluorescence
[en] Rats
[en] Surgical
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/166189
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