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In vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A)

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In vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A)

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Título In vitro characterization of GE negative bovine herpesvirus types 1.1 (BHV-1.1) and 1.2A (BHV-1.2A)
Autor Spilki, Fernando Rosado
Franco, Ana Claudia
Rijsewijk, Franciscus Antonius Maria
Weiblen, Rudi
Flores, Eduardo Furtado
Roehe, Paulo Michel
Abstract This study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion in the glycoprotein E gene (BHV-1.2a gE-). The plaque sizes, penetration and growth kinetics of the Brazilian BHV-1.2a gE- were studied and compared with the parental virus, as well as with a BHV-1.1 gE- recombinant derived from an European BHV-1.1 strain. No statistical differences were observed between the gE- recombinants and the respective parental viruses penetration assays were performed. When single step growth curves were studied, no statistical differences were observed between gE- and parental viruses. However, it was observed that both gE- viruses were excreted from cells in significantly higher titres at 11 hours post infection in comparison with parental viruses. No statistical differences were observed when plaque sizes of parental viruses or gE- viruses we analyzed separately in each cell type. However, both gErecombinants displayed a significantly reduced plaque areas on three different cell cultures, in comparison with parental viruses, indicating that the lack of gE had the same effect on both BHV-1 subtypes, manifested by a restricted cell-to-cell spread in infected cells.
Contido em Brazilian journal of microbiology. São Paulo, SP. Vol. 35, n. 3 (Jul./Set. 2004), p. 264-268
Assunto Herpesvírus bovino
[en] BHV-1.2a
[en] Bovine herpesviruses
[en] Differential vaccine
[en] gE- recombinant
[en] Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis
[en] In vitro growth characteristics
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/20287
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