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Evaluation of an indirect Elisa for the detection of Salmonella in chicken meat

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Evaluation of an indirect Elisa for the detection of Salmonella in chicken meat

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Título Evaluation of an indirect Elisa for the detection of Salmonella in chicken meat
Autor Schneid, Andréa dos Santos
Rodrigues, Kelly Lameiro
Chemello, Davi
Tondo, Eduardo Cesar
Ayub, Marco Antônio Záchia
Aleixo, José Antônio Guimarães
Abstract In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonella enterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of 82% and 98% respectively. The serotyping of the isolates revealed 31 Salmonella enterica serovar Enteritidis, 2 Salmonella enterica serovar Heidelberg, 1 Salmonella enterica serovar Choleraesuis and 1 Salmonella enterica sorovar 6,7
Contido em Brazilian journal of microbiology. São Paulo. vol. 37, n. 3 (jul/set. 2006), p. 350-355
Assunto Anticorpo monoclonal
Carne de frango
ELISA
Salmonella
[en] ELISA
[en] Monoclonal antibody
[en] Policlonal antibody
[en] Salmonella
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/20395
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