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Keratinolytic bacteria isolated from feather waste

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Keratinolytic bacteria isolated from feather waste

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Título Keratinolytic bacteria isolated from feather waste
Autor Riffel, Alessandro
Brandelli, Adriano
Abstract The aim of this study was to characterize keratinolytic bacteria isolated from feather waste. Four isolates were selected after growth on solid medium with feather meal as sole carbon and nitrogen source and screened for proteolytic activity on milk agar plates. Three isolates were Gram-negative (belonged to the genera Burkholderia, Chryseobacterium and Pseudomonas) and one was Gram-positive (Microbacterium sp.). These bacteria grew on diverse keratin wastes such as feather meal, raw feathers, chicken nails, hair and wool. Keratinase activity was detected during growth, but the complete degradation of these substrates was not always achieved. The proteolytic character of crude enzymes was assessed using azokeratin and azocasein as substrates. The keratinases were active on both substrates and were similar in keratin hydrolysis when compared with commercially available microbial peptidases. These novel keratinolytic isolates have potential biotechnological use in processes involving keratin hydrolysis.
Contido em Brazilian journal of microbiology. São Paulo. vol. 37, n. 3 (jul./set. 2006), p. 395-399
Assunto Bactérias queratinolíticas
Biodegradação
Farinha de pena
Pena de aves
Protease
Proteólise
Queratinase
Resíduo orgânico
[en] Feather
[en] Keratin
[en] Peptidase
[en] Proteolysis
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/20396
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