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dc.contributor.authorVerdin, Sylvia Elisa Frizzopt_BR
dc.contributor.authorSilva, Silvia Regina Pavan dapt_BR
dc.contributor.authorPereira, Dariane Castropt_BR
dc.contributor.authorSchatkoski, Aline Modelskipt_BR
dc.contributor.authorCorção, Gertrudespt_BR
dc.date.accessioned2010-04-16T09:15:27Zpt_BR
dc.date.issued2007pt_BR
dc.identifier.issn1678-0345pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/20558pt_BR
dc.description.abstractMinimally processed vegetables go through many steps before they are refrigerated, selection, washing, peeling, cutting, disinfection and finally packaging. However, if no care is taken at the origin of the raw materials and in the processing stages, there is a chance of finding pathogenic bacteria, such as Listeria monocytogenes, which are able to grow at low temperatures. The aim of this research was to verify the occurrence of Listeria sp. in minimally processed vegetables sold in Porto Alegre, Brazil, and the genetic relationship among the isolates. Minimally processed salads were sampled monthly from local supermarkets and analyzed by inoculation on Listeria Enrichment Broth and subsequent seeding on two selective media, Palcam and Modified Oxford Agar. The typical colonies were identified to species level and their intergenic region 16S-23S rDNA were amplified in order to verify the genetic variability. Species of Listeria were found in 23 of the 52 processed salad samples analyzed and L. monocytogenes was found in seven. The presence of L. monocytogenes in the samples is a health concern, as these salads are eaten without further treatment by the consumer. The amplification of the intergenic region 16S-23S rDNA, showed a great genetic diversity among the isolates, with 43 different patterns, proving the usefulness of this technique in epidemiologic studies.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofActa scientiae veterinariae. Porto Alegre, RS. Vol. 35, n. 2 (2007), pub. 722, p. 167-172.pt_BR
dc.rightsOpen Accessen
dc.subjectListeriaen
dc.subjectVegetais minimamente processadospt_BR
dc.subjectMinimally processeden
dc.subjectListeria monocytogenespt_BR
dc.subjectContaminação de alimentospt_BR
dc.subject16S-23S rDNASen
dc.subjectSpacer regionen
dc.subjectVegetablesen
dc.titleOccurrence and genetic characterization of Listeria spp. in minimally processed vegetables commercialized in Porto Alegre, Brazilpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000633831pt_BR
dc.type.originNacionalpt_BR


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