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In vitro activity of Origanum vulgare essential oil against Candida species

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In vitro activity of Origanum vulgare essential oil against Candida species

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Título In vitro activity of Origanum vulgare essential oil against Candida species
Autor Cleff, Marlete Brum
Meinerz, Ana Raquel Mano
Xavier, Melissa Orzechowski
Schuch, Luiz Filipe Damé
Meireles, Mário Carlos Araújo
Rodrigues, Maria Regina Alves
Mello, Joao Roberto Braga de
Abstract The aim of this study was to evaluate the in vitro activity of the essential oil extracted from Origanum vulgare against sixteen Candida species isolates. Standard strains tested comprised C. albicans (ATCC strains 44858, 4053, 18804 and 3691), C. parapsilosis (ATCC 22019), C. krusei (ATCC 34135), C. lusitaniae (ATCC 34449) and C. dubliniensis (ATCC MY646). Six Candida albicans isolates from the vaginal mucous membrane of female dogs, one isolate from the cutaneous tegument of a dog and one isolate of a capuchin monkey were tested in parallel. A broth microdilution technique (CLSI) was used, and the inoculum concentration was adjusted to 5 x 106 CFU mL-1. The essential oil was obtained by hydrodistillation in a Clevenger apparatus and analyzed by gas chromatography. Susceptibility was expressed as Minimal Inhibitory Concentration (MIC) and Minimal Fungicidal Concentration (MFC) All isolates tested in vitro were sensitive to O. vulgare essential oil. The chromatographic analysis revealed that the main compounds present in the essential oil were 4-terpineol (47.95%), carvacrol (9.42%), thymol (8.42%) and -terpineol (7.57%). C. albicans isolates obtained from animal mucous membranes exhibited MIC and MFC values of 2.72 L mL-1 and 5 L mL-1, respectively. MIC and MFC values for C. albicans standard strains were 2.97 L mL-1 and 3.54 L mL-1, respectively. The MIC and MFC for non-albicans species were 2.10 L mL-1 and 2.97 L mL-1, respectively. The antifungal activity of O. vulgare essential oil against Candida spp. observed in vitro suggests its administration may represent an alternative treatment for candidiasis.
Contido em Brazilian journal of microbiology. São Paulo. Vol. 41, no. 1 (Jan./Mar. 2010), p. 116-123
Assunto Candida
In vitro
Oleos essenciais
Origanum vulgare
[en] Candida
[en] Essential oil
[en] In vitro
[en] Origanum vulgare
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/20911
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