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Partial characterization of nif genes from the bacterium Azospirillum amazonense

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Partial characterization of nif genes from the bacterium Azospirillum amazonense

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Título Partial characterization of nif genes from the bacterium Azospirillum amazonense
Autor Potrich, Deise Porto
Passaglia, Luciane Maria Pereira
Schrank, Irene Silveira
Abstract Azospirillum amazonense revealed genomic organization patterns of the nitrogen fixation genes similar to those of the distantly related species A. brasilense. Our work suggests that A. brasilense nifHDK, nifENX, fixABC operons and nifA and glnB genes may be structurally homologous to the counterpart genes of A. amazonense. This is the first analysis revealing homology between A. brasilense nif genes and the A. amazonense genome. Sequence analysis of PCR amplification products revealed similarities between the amino acid sequences of the highly conserved nifD and glnB genes of A. amazonense and related genes of A. brasilense and other bacteria. However, the A. amazonense non-coding regions (the upstream activator sequence region and the region between the nifH and nifD genes) differed from related regions of A. brasilense even in nitrogenase structural genes which are highly conserved among diazotrophic bacteria. The feasibility of the 16S ribosomal RNA gene-based PCR system for specific detection of A. amazonense was shown. Our results indicate that the PCR primers for 16S rDNA defined in this article are highly specific to A. amazonense and can distinguish this species from A. brasilense.
Contido em Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 9 (Sept. 2001), p. 1105-1113
Assunto Azospirillum amazonense
Biologia molecular
DNA
Reação em cadeia da polimerase
RNA
[en] 16S rDNA
[en] GlnB
[en] Nif genes
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/21128
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