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dc.contributor.authorHermel, Erica do Espirito Santopt_BR
dc.contributor.authorSeverino, Gabriela Sentenapt_BR
dc.contributor.authorCecconello, Ana Lúciapt_BR
dc.contributor.authorPereira, Francine Martinspt_BR
dc.contributor.authorSanvitto, Gilberto Luizpt_BR
dc.contributor.authorLucion, Aldo Boltenpt_BR
dc.date.accessioned2010-04-24T04:15:26Zpt_BR
dc.date.issued2001pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/21129pt_BR
dc.description.abstractNeonatal handling has long-lasting effects on behavior and stress reactivity. The purpose of the present study was to investigate the effect of neonatal handling on the number of dopaminergic neurons in the hypothalamic nuclei of adult male rats as part of a series of studies that could explain the long-lasting effects of neonatal stimulation. Two groups of Wistar rats were studied: nonhandled (pups were left undisturbed, control) and handled (pups were handled for 1 min once a day during the first 10 days of life). At 75-80 days, the males were anesthetized and the brains were processed for immunohistochemistry. An anti-tyrosine hydroxylase antibody and the avidin-biotinperoxidase method were used. Tyrosine hydroxylase-immunoreactive (TH-IR) neurons were counted bilaterally in the arcuate, paraventricular and periventricular nuclei of the hypothalamus in 30-μm sections at 120-μm intervals. Neonatal handling did not change the number of TH-IR neurons in the arcuate (1021 ± 206, N = 6; 1020 ± 150, N = 6; nonhandled and handled, respectively), paraventricular (584 ± 85, N = 8; 682 ± 62, N = 9) or periventricular (743 ± 118, N = 7; 990 ± 158, N = 7) nuclei of the hypothalamus. The absence of an effect on the number of dopaminergic cells in the hypothalamus indicates that the reduction in the amount of neurons induced by neonatal handling, as shown by other studies, is not a general phenomenon in the brain.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research =Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 9 (Sept. 2001), p. 1191-1195pt_BR
dc.rightsOpen Accessen
dc.subjectStressen
dc.subjectFisiologiapt_BR
dc.subjectNeonatal stimulationen
dc.subjectDopamineen
dc.subjectImmunohistochemistryen
dc.subjectHypothalamusen
dc.subjectTyrosine hydroxylaseen
dc.titleNeonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male ratspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000324845pt_BR
dc.type.originNacionalpt_BR


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