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Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices

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Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices

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Título Effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices
Autor Torres, Iraci Lucena da Silva
Gamaro, Giovana Duzzo
Cucco, Simone Nascimento Silveira
Michalowski, Mariana Bohns
Correa, Jaqueline Betina Broenstrup
Perry, Marcos Luiz Santos
Dalmaz, Carla
Abstract It has been suggested that glucocorticoids released during stress might impair neuronal function by decreasing glucose uptake by hippocampal neurons. Previous work has demonstrated that glucose uptake is reduced in hippocampal and cerebral cortex slices 24 h after exposure to acute stress, while no effect was observed after repeated stress. Here, we report the effect of acute and repeated restraint stress on glucose oxidation to CO2 in hippocampal and cerebral cortex slices and on plasma glucose and corticosterone levels. Male adult Wistar rats were exposed to restraint 1 h/day for 50 days in the chronic model. In the acute model there was a single exposure. Immediately or 24 h after stress, the animals were sacrificed and the hippocampus and cerebral cortex were dissected, sliced, and incubated with Krebs buffer, pH 7.4, containing 5 mM glucose and 0.2 µCi D-[U-14C] glucose. CO2 production from glucose was estimated. Trunk blood was also collected, and both corticosterone and glucose were measured. The results showed that corticosterone levels after exposure to acute restraint were increased, but the increase was smaller when the animals were submitted to repeated stress. Blood glucose levels increased after both acute and repeated stress. However, glucose utilization, measured as CO2 production in hippocampal and cerebral cortex slices, was the same in stressed and control groups under conditions of both acute and chronic stress. We conclude that, although stress may induce a decrease in glucose uptake, this effect is not sufficient to affect the energy metabolism of these cells.
Contido em Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 1 (Jan. 2001), p. 111-116
Assunto Bioquímica
[en] Cerebral cortex
[en] Chronic stress
[en] CO2 production
[en] Glucose oxidation
[en] Hippocampus
[en] Restraint stress
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/21133
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