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dc.contributor.authorBrusque, Ana Mariapt_BR
dc.contributor.authorRotta, Liane Nancipt_BR
dc.contributor.authorPettenuzzo, Letícia Ferreirapt_BR
dc.contributor.authorJunqueira, Deborapt_BR
dc.contributor.authorSchwarzbold, Carolina Vargaspt_BR
dc.contributor.authorWyse, Angela Terezinha de Souzapt_BR
dc.contributor.authorWannmacher, Clovis Milton Duvalpt_BR
dc.contributor.authorDutra Filho, Carlos Severopt_BR
dc.contributor.authorWajner, Moacirpt_BR
dc.date.accessioned2010-04-24T04:15:27Zpt_BR
dc.date.issued2001pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/21136pt_BR
dc.description.abstractLevels of methylmalonic acid (MMA) comparable to those of human methylmalonic acidemia were achieved in blood (2-2.5 mmol/l) and brain (1.35 µmol/g) of rats by administering buffered MMA, pH 7.4, subcutaneously twice a day from the 5th to the 28th day of life. MMA doses ranged from 0.76 to 1.67 µmol/g as a function of animal age. Control rats were treated with saline in the same volumes. The animals were sacrificed by decapitation on the 28th day of age. Blood was taken and the brain was rapidly removed. Medulla, pons, the olfactory lobes and cerebellum were discarded and the rest of the brain "cerebrum" was isolated. Body and "cerebrum" weight were measured, as well as the cholesterol and triglyceride concentrations in blood and the content of myelin, total lipids, and the concentrations of the lipid fractions (cholesterol, glycerolipids, phospholipids and ganglioside N-acetylneuraminic acid (ganglioside-NANA) in the "cerebrum". Chronic MMA administration had no effect on body or "cerebrum" weight, suggesting that the metabolites per se neither affect the appetite of the rats nor cause malnutrition. In contrast, MMA caused a significant reduction of plasma triglycerides, but not of plasma cholesterol levels. A significant diminution of myelin content and of ganglioside- NANA concentration was also observed in the "cerebrum". We propose that the reduction of myelin content and ganglioside- NANA caused by MMA may be related to the delayed myelination/ cerebral atrophy and neurological dysfunction found in methylmalonic acidemic children.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 2 (Feb. 2001), p. 227-231pt_BR
dc.rightsOpen Accessen
dc.subjectBioquímicapt_BR
dc.subjectMethylmalonic aciden
dc.subjectGlyceridesen
dc.subjectLipidsen
dc.subjectMyelinen
dc.subjectRat brainen
dc.subjectMethylmalonic acidemiaen
dc.titleChronic postnatal administration of methylmalonic acid provokes a decrease of myelin content and ganglioside N-acetylneuramic acid concentration in cerebrum of young ratspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000327067pt_BR
dc.type.originNacionalpt_BR


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