Repositório Digital

A- A A+

Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neolycoprotein

.

Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neolycoprotein

Mostrar registro completo

Estatísticas

Título Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neolycoprotein
Autor Passos, Eduardo Pandolfi
Brugnara, Laura
Facin, Andrea Cintra
Riffel, Alessandro
Lima, Geraldo Rodrigues de
Freitas, V.
Brandelli, Adriano
Abstract The effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophoretreated sperm showed that NGP-induced exocytosis differed significantly between the PFc (11%) and PFe/PFi groups (17%), and the ionophore-induced AR was higher for PFi (33%) than PFc/PFe (25%). The incidence of the NGP-induced AR was reduced in the first hour of pre-incubation with PFc and remained nearly constant throughout 4 h of incubation. The present data indicate that PF possesses a protective factor which prevents premature AR.
Contido em Brazilian journal of medical and biological research. Ribeirão Preto, SP. Vol. 32, no. 1 (Jan. 1999), p. 59-65
Assunto Endometriose
Exocitose
Fluido peritoneal
Infertilidade
N-acetilglucosaminidase
Reação acrossômica
[en] Acrosome reaction
[en] Endometriosis
[en] Infertility
[en] Peritoneal fluid
[en] Sperm
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/21153
Arquivos Descrição Formato
000294542.pdf (119.9Kb) Texto completo Adobe PDF Visualizar/abrir

Este item está licenciado na Creative Commons License

Este item aparece na(s) seguinte(s) coleção(ões)


Mostrar registro completo

Percorrer



  • O autor é titular dos direitos autorais dos documentos disponíveis neste repositório e é vedada, nos termos da lei, a comercialização de qualquer espécie sem sua autorização prévia.
    Projeto gráfico elaborado pelo Caixola - Clube de Criação Fabico/UFRGS Powered by DSpace software, Version 1.8.1.