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dc.contributor.authorGimba, Etel Rodrigues Pereirapt_BR
dc.contributor.authorChemale, Gustavopt_BR
dc.contributor.authorFarias, Sandra Estrazulaspt_BR
dc.contributor.authorZaha, Arnaldopt_BR
dc.date.accessioned2010-04-24T04:15:32Zpt_BR
dc.date.issued2000pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/21159pt_BR
dc.description.abstractWe report here for the first time the structure and function of a promoter from a cestode. The ability of DNA fragments respectively encompassing the 935-bp and 524-bp regions upstream from the ATG codon from the EgactI and EgactII actin genes of Echinococcus granulosus to promote transcription was studied in the NIH3T3 mouse cell line. The results of transfection assays showed that both regions have strong promoter activity in these cells. The fragments were tested in both orientations and the 524-bp fragment of EgactII presented a bidirectional promoter activity. Deletion analysis of EgactI and EgactII promoters indicated the presence of regulatory regions containing putative silencer elements. These results indicate that both EgactI and EgactII promoters are functional and that the preliminary functional evaluation of E. granulosus and possibly of other cestode promoters can be performed in heterologous cell lines.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 33, no. 11 (Nov. 2000), p. 1283-1292pt_BR
dc.rightsOpen Accessen
dc.subjectEchinococcus granulosusen
dc.subjectGenéticapt_BR
dc.subjectActinen
dc.subjectPromotersen
dc.subjectCestodeen
dc.subjectGene expressionen
dc.subjectDeletionsen
dc.titleCloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell linept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000296773pt_BR
dc.type.originNacionalpt_BR


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