Repositório Digital

A- A A+

Retinol-induced changes in the phosphorylation levels of histones and high mobility group proteins from sertoli cells

.

Retinol-induced changes in the phosphorylation levels of histones and high mobility group proteins from sertoli cells

Mostrar registro completo

Estatísticas

Título Retinol-induced changes in the phosphorylation levels of histones and high mobility group proteins from sertoli cells
Autor Moreira, Jose Claudio Fonseca
Dal Pizzol, Felipe
Rocha, Adriana Brondani da
Klamt, Fabio
Ribeiro, Nede Carlos
Ferreira, Carlos Jose Sarmento
Bernard, Elena Aida
Abstract Chromatin proteins play a role in the organization and functions of DNA. Covalent modifications of nuclear proteins modulate their interactions with DNA sequences and are probably one of the multiple factors involved in the process of switch on/off transcriptionally active regions of DNA. Histones and high mobility group proteins (HMG) are subject to many covalent modifications that may modulate their capacity to bind to DNA. We investigated the changes induced in the phosphorylation pattern of cultured Wistar rat Sertoli cell histones and high mobility group protein subfamilies exposed to 7 µM retinol for up to 48 h. In each experiment, 6 h before the end of the retinol treatment each culture flask received 370 KBq/ml [32P]-phosphate. The histone and HMGs were isolated as previously described [Moreira et al. Medical Science Research (1994) 22: 783-784]. The total protein obtained by either method was quantified and electrophoresed as described by Spiker [Analytical Biochemistry (1980) 108: 263-265]. The gels were stained with Coomassie brilliant blue R-250 and the stained bands were cut and dissolved in 0.5 ml 30% H2O2 at 60oC for 12 h. The vials were chilled and 5.0 ml scintillation liquid was added. The radioactivity in each vial was determined with a liquid scintillation counter. Retinol treatment significantly changed the pattern of each subfamily of histone and high mobility group proteins.
Contido em Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 33, no. 3 (Mar. 2000), p. 287-293
Assunto Bioquímica
[en] High mobility group proteins
[en] Histones
[en] Phosphorylation
[en] Retinol
[en] Sertoli cells
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/21160
Arquivos Descrição Formato
000297137.pdf (154.0Kb) Texto completo (inglês) Adobe PDF Visualizar/abrir

Este item está licenciado na Creative Commons License

Este item aparece na(s) seguinte(s) coleção(ões)


Mostrar registro completo

Percorrer



  • O autor é titular dos direitos autorais dos documentos disponíveis neste repositório e é vedada, nos termos da lei, a comercialização de qualquer espécie sem sua autorização prévia.
    Projeto gráfico elaborado pelo Caixola - Clube de Criação Fabico/UFRGS Powered by DSpace software, Version 1.8.1.