Repositório Digital

A- A A+

Glutathione peroxidase induction protects Saccharomyces cerevisiae sod1deltasod2delta double mutants against oxidative damage

.

Glutathione peroxidase induction protects Saccharomyces cerevisiae sod1deltasod2delta double mutants against oxidative damage

Mostrar registro completo

Estatísticas

Título Glutathione peroxidase induction protects Saccharomyces cerevisiae sod1deltasod2delta double mutants against oxidative damage
Autor Manfredini, Vanusa
Roehrs, Rafael
Peralba, Maria do Carmo Ruaro
Henriques, Joao Antonio Pegas
Saffi, Jenifer
Ramos, Ana Ligia Lia de Paula
Benfato, Mara da Silveira
Abstract Saccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1∆, sod2∆and the double mutant) were subjected to H2O2 stress in the stationary phase. The highest sensitivity was observed in the sod2∆mutant, while the sod1∆sod2∆double mutant was not sensitive. sod mutants had lower catalase activity (44%) than wildtype cells, independent of H2O2 stress. Untreated cells of sod1∆sod2∆ double mutants showed increased glutathione peroxidase activity (126%), while sod1∆had lower activity (77%) than the wild type. Glutathione levels in sod1∆were increased (200-260%) after exposure to various H2O2 concentrations. In addition, the highest malondialdehyde levels could be observed without H2O2 treatment in sod1∆ (167%) and sod2∆(225%) mutants. In contrast, the level of malondialdehyde in the sod1∆sod2∆double mutant was indistinguishable from that of the wild type. These results suggest that resistance to H2O2 by sod1∆sod2∆cells depends on the induction of glutathione peroxidase and is independent of catalase, and that glutathione is a primary antioxidant in the defense against H2O2 in stationary phase sod1∆ mutants.
Contido em Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 37, no. 2 (2004), p. 159-165
Assunto Catalase
Espécies reativas de oxigênio
Glutationa
Peroxido de hidrogenio
Saccharomyces cerevisiae : Gene : Mutacao
Superóxido dismutase
[en] Catalase
[en] Glutathione
[en] Hydrogen peroxide
[en] Reactive oxygen species
[en] Saccharomyces cerevisiae
[en] Superoxide dismutase
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/21176
Arquivos Descrição Formato
000406308.pdf (480.4Kb) Texto completo (inglês) Adobe PDF Visualizar/abrir

Este item está licenciado na Creative Commons License

Este item aparece na(s) seguinte(s) coleção(ões)


Mostrar registro completo

Percorrer



  • O autor é titular dos direitos autorais dos documentos disponíveis neste repositório e é vedada, nos termos da lei, a comercialização de qualquer espécie sem sua autorização prévia.
    Projeto gráfico elaborado pelo Caixola - Clube de Criação Fabico/UFRGS Powered by DSpace software, Version 1.8.1.