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dc.contributor.authorFreitas, Ana Lucia Peixoto dept_BR
dc.contributor.authorBarth, Afonso Luispt_BR
dc.date.accessioned2010-04-24T04:15:39Zpt_BR
dc.date.issued2004pt_BR
dc.identifier.issn0100-879Xpt_BR
dc.identifier.urihttp://hdl.handle.net/10183/21191pt_BR
dc.description.abstractTyping techniques are essential for understanding hospital epidemiology, permitting the elucidation of the source of infection and routes of bacterial transmission. Although DNA-based techniques are the “gold standard” for the epidemiological study of Pseudomonas aeruginosa, antibiotic profiles and biochemical results are used because they are easy to perform and to interpret and relatively inexpensive. Antibiotypes (susceptibility profiles) and biotypes (biochemical profiles) were compared to genotypes established by DNA restriction enzyme analysis in 81 clinical isolates of P. aeruginosa from three hospitals in Porto Alegre, Brazil. The epidemiological relationship among patients was also evaluated. Susceptibility and restriction profiles were discrepant in more than 50% of the cases, and many antibiotypes were observed among isolates from the same genotype. Furthermore, susceptibility profiles did not allow the distinction of isolates from unrelated genotypes. Since a large number of isolates (63%) yielded the same biochemical results, only 10 biotypes were detected, showing that this typing method has a low discriminatory power. On the other hand, DNA restriction enzyme typing allowed us to establish 71 distinct types. Epidemiological data about the relation among P. aeruginosa isolates were not conclusive. The results of the present study indicate that the only method that can establish a clonal relation is DNA restriction enzyme typing, whereas the other methods may cause misleading interpretations and are inadequate to guide proper infection control measures.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 37, no. 1 (Jan. 2004), p.77-82pt_BR
dc.rightsOpen Accessen
dc.subjectPseudomonas aeruginosaen
dc.subjectPseudomonas aeruginosapt_BR
dc.subjectTypingen
dc.subjectGenotypingen
dc.subjectPhenotypic markersen
dc.titleTyping of Pseudomonas aeruginosa from hospitalized patients : a comparison of susceptibility and biochemical profiles with genotypept_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000435655pt_BR
dc.type.originNacionalpt_BR


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