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Peripheral markers of oxidative stress in chronic mercuric chloride intoxication

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Peripheral markers of oxidative stress in chronic mercuric chloride intoxication

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Título Peripheral markers of oxidative stress in chronic mercuric chloride intoxication
Autor Gutierrez, Lucila Ludmila Paula
Mazzotti, Nicolle Gollo
Araújo, Alex Sander da Rosa
Klipel, Rodrigo Blauth
Belló-Klein, Adriane
Fernandes, Tania Regina Gattelli
Llesuy, Susana Francisca
Abstract The present study was designed to evaluate the time course changes in peripheral markers of oxidative stress in a chronic HgCl2 intoxication model. Twenty male adult Wistar rats were treated subcutaneously daily for 30 days and divided into two groups of 10 animals each: Hg, which received HgCl2 (0.16 mg kg-1 day-1), and control, receiving the same volume of saline solution. Blood was collected at the first, second and fourth weeks of Hg administration to evaluate lipid peroxidation (LPO), total radical trapping antioxidant potential (TRAP), and superoxide dismutase (Cu,Zn-SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and catalase (CAT). HgCl2 administration induced a rise (by 26%) in LPO compared to control (143 ± 10 cps/mg hemoglobin) in the second week and no difference was found at the end of the treatment. At that time, GST and GPx were higher (14 and 24%, respectively) in the Hg group, and Cu,Zn-SOD was lower (54%) compared to control. At the end of the treatment, Cu,Zn-SOD and CAT were higher (43 and 10%, respectively) in the Hg group compared to control (4.6 ± 0.3 U/mg protein; 37 ± 0.9 pmol/mg protein, respectively). TRAP was lower (69%) in the first week compared to control (43.8 ± 1.9 mM Trolox). These data provide evidence that HgCl2 administration is accompanied by systemic oxidative damage in the initial phase of the process, which leads to adaptive changes in the antioxidant reserve, thus decreasing the oxidative injury at the end of 30 days of HgCl2 administration. These results suggest that a preventive treatment with antioxidants would help to avoid oxidative damage in subjects with chronic intoxication.
Contido em Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 39, n. 6 (jun. 2006), p. 767-772
Assunto Antioxidantes
Estresse oxidativo
[en] Antioxidant enzymes
[en] Heavy metals
[en] HgCl2
[en] Lipid peroxidation
[en] Total radical-trapping antioxidant potential
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/21210
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