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Neutrophil apoptosis : a marker of disease severity in sepsis and sepsis-induced acute respiratory distress syndrome

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Neutrophil apoptosis : a marker of disease severity in sepsis and sepsis-induced acute respiratory distress syndrome

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Título Neutrophil apoptosis : a marker of disease severity in sepsis and sepsis-induced acute respiratory distress syndrome
Autor Fialkow, Léa
Bozzetti, Mary Clarisse
Fochesatto Filho, Luciano
Rodrigues Filho, Edison Moraes
Ladniuk, Roberta Maboni
Milani, Adriana Rosa
Pierozan, Paula
Prolla, João Carlos
Downey, Gregory P.
Moura, Rafael Moraes de
Vachon, Eric
Abstract Introduction Apoptosis of neutrophils (polymorphonuclear neutrophils [PMNs]) may limit inflammatory injury in sepsis and acute respiratory distress syndrome (ARDS), but the relationship between the severity of sepsis and extent of PMN apoptosis and the effect of superimposed ARDS is unknown. The objective of this study was to correlate neutrophil apoptosis with the severity of sepsis and sepsis-induced ARDS. Methods A prospective cohort study was conducted in intensive care units of three tertiary hospitals in Porto Alegre, southern Brazil. Fifty-seven patients with sepsis (uncomplicated sepsis, septic shock, and sepsis-induced ARDS) and 64 controls were enrolled. Venous peripheral blood was collected from patients with sepsis within 24 hours of diagnosis. All surgical groups, including controls, had their blood drawn 24 hours after surgery. Control patients on mechanical ventilation had blood collected within 24 hours of initiation of mechanical ventilation. Healthy controls were blood donors. Neutrophils were isolated, and incubated ex vivo, and apoptosis was determined by light microscopy on cytospun preparations. The differences among groups were assessed by analysis of variance with Tukeys. Results In medical patients, the mean percentage of neutrophil apoptosis (± standard error of the mean [SEM]) was lower in sepsis-induced ARDS (28% ± 3.3%; n = 9) when compared with uncomplicated sepsis (57% ± 3.2%; n = 8; p < 0.001), mechanical ventilation without infection, sepsis, or ARDS (53% ± 3.0%; n = 11; p < 0.001) and healthy controls (69% ± 1.1%; n = 33; p < 0.001) but did not differ from septic shock (38% ± 3.7%; n = 12; p = 0.13). In surgical patients with sepsis, the percentage of neutrophil apoptosis was lower for all groups when compared with surgical controls (52% ± 3.6%; n = 11; p < 0.001). Conclusion In medical patients with sepsis, neutrophil apoptosis is inversely proportional to the severity of sepsis and thus may be a marker of the severity of sepsis in this population.
Contido em Critical care. London. Vol. 10, no. 6 (2006), p. 1-14
Assunto Apoptose
Neutrófilos
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/21724
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