Repositório Digital

A- A A+

An effective device for generating alginate microcapsules

.

An effective device for generating alginate microcapsules

Mostrar registro completo

Estatísticas

Título An effective device for generating alginate microcapsules
Autor Bressel, Tatiana Azevedo Bastian
Paz, Ana Helena da Rosa
Baldo, Guilherme
Cirne Lima, Elizabeth Obino
Matte, Ursula da Silveira
Pereira, Maria Luiza Saraiva
Abstract An alternative approach to somatic gene therapy is to deliver the therapeutic protein by implanting genetically modified cells that could overexpress the gene of interest. Microencapsulation devices were designed to protect cells from host rejection and prevent the foreign cells from spreading while allowing protein secretion. Alginate microcapsules form a semi-permeable structure that is suitable for in vivo injection. In this study, we report an effective laboratory protocol for producing calcium alginate microcapsules, following optimization of uniformly shaped and sized particles containing viable cells. Encapsulation of baby hamster kidney (BHK) cells in alginate microcapsules was performed using a simple device consisting of a cylinder of compressed air and a peristaltic pump. A cell suspension flow of 100 mL h-1 and an air jet flow of 10 L min-1 produced the best uniformity of microcapsule size and shape. Cells maintained viability in culture for 4 weeks without any signs of necrosis, and protein diffusion was observed during this period. Our results clearly demonstrated that microisolation of BHK cells in alginate using a simple assembly device could provide an environment that is capable of preserving live cells. In addition, encapsulated cells under the conditions described were able to secrete an active enzyme even after four weeks, thus becoming potentially compatible with therapeutic protein delivery.
Contido em Genetics and molecular biology. Ribeirão Preto. Vol. 31, no. 1 (2008), p. 136-140
Assunto Bioquímica
Genética
Microencapsulação
Microesferas
[en] Alginate
[en] Beads
[en] Controlled release
[en] Drug delivery
[en] Microcapsules
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/23410
Arquivos Descrição Formato
000686501.pdf (127.6Kb) Texto completo (inglês) Adobe PDF Visualizar/abrir

Este item está licenciado na Creative Commons License

Este item aparece na(s) seguinte(s) coleção(ões)


Mostrar registro completo

Percorrer



  • O autor é titular dos direitos autorais dos documentos disponíveis neste repositório e é vedada, nos termos da lei, a comercialização de qualquer espécie sem sua autorização prévia.
    Projeto gráfico elaborado pelo Caixola - Clube de Criação Fabico/UFRGS Powered by DSpace software, Version 1.8.1.