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dc.contributor.authorBender, Eduardo Andrépt_BR
dc.contributor.authorFreitas, Ana Lucia Peixoto dept_BR
dc.contributor.authorReiter, Keli Cristinept_BR
dc.contributor.authorLutz, Larissapt_BR
dc.contributor.authorBarth, Afonso Luispt_BR
dc.date.accessioned2010-06-19T04:17:48Zpt_BR
dc.date.issued2009pt_BR
dc.identifier.issn1517-8382pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/23958pt_BR
dc.description.abstractIn the past two decades the members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. In the present study, we evaluated the antimicrobial resistance and genotypic characteristics of 203 Enterococcus spp. recovered from different clinical sources from two hospitals in Porto Alegre, Rio Grande do Sul, Brazil. The species were identified by conventional biochemical tests and by an automated system. The genetic diversity of E. faecalis presenting high-level aminoglycoside resistance (HLAR) was assessed by pulsed-field gel electrophoresis of chromosomal DNA after SmaI digestion. The E. faecalis was the most frequent specie (93.6%), followed by E. faecium (4.4%). The antimicrobial resistance profile was: 2.5% to ampicillin, 0.5% to vancomycin, 0.5% teicoplanin, 33% to chloramphenicol, 2% to nitrofurantoin, 66.1% to erythromycin, 66.5% to tetracycline, 24.6% to rifampicin, 30% to ciprofloxacin and 87.2% to quinupristin-dalfopristin. A total of 10.3% of the isolates proved to be HLAR to both gentamicin and streptomycin (HLR-ST/GE), with 23.6% resistant only to gentamicin (HLR-GE) and 37.4% only to streptomycin (HLR-ST). One predominant clonal group was found among E. faecalis HLR-GE/ST. The prevalence of resistance among beta-lactam antibiotics and glycopeptides was very low. However, in this study there was an increased number of HLR Enterococcus which may be spreading intra and inter-hospital.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofBrazilian journal of microbiology. São Paulo, SP. Vol. 40, n. 3 (set. 2009), p. 693-700pt_BR
dc.rightsOpen Accessen
dc.subjectEnterococcusen
dc.subjectEnterococcuspt_BR
dc.subjectResistência bacterianapt_BR
dc.subjectResistanceen
dc.subjectHLARen
dc.subjectPFGEen
dc.titleIdentification, antimicrobial resistance and genotypic characterization of enterococcus spp. isolated in Porto Alegre, Brazilpt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000710305pt_BR
dc.type.originNacionalpt_BR


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