Repositório Digital

A- A A+

High polymorphism in genes encoding antigen B from human infecting strains of Echinococcus granulosus

.

High polymorphism in genes encoding antigen B from human infecting strains of Echinococcus granulosus

Mostrar registro completo

Estatísticas

Título High polymorphism in genes encoding antigen B from human infecting strains of Echinococcus granulosus
Autor Kamenetzky, Laura
Muzulin, P. M.
Gutierrez, Ariana M.
Angel, S.O.
Zaha, Arnaldo
Guarnera, Eduardo A.
Rosenzvit, Mara Cecília
Abstract Echinococcus granulosus antigen B (AgB) is encoded by a gene family and is involved in the evasion of the host immune response. E. granulosus exists as a number of strains (G1–G10) that differ in biological characteristics. We used PCR-SSCP followed by DNA sequencing to evaluate sequence variation and transcription profile of AgB in 5 E. granulosus strains#. Twenty-four genomic sequences were isolated and clustered in 3 groups related to 2 of the 5 reported AgB genes. AgB4 genes were present in almost all strains, whereas AgB2 were present as functional genes exclusively in G1/G2 cluster, and as non-functional genes in G5 and the G6/G7 cluster, suggesting inter-strain variation The AgB transcription patterns, analysed by RT-PCR, showed that AgB2 and AgB4 genes were transcribed in G1, while only the AgB4 gene was transcribed in G7 strain. Cysts from the same strain or cluster shared more genomic and cDNA variants than cysts from different strain or cluster. The level of nucleotide and deduced amino acid sequence variation observed is higher than that reported so far for coding genes of other helminths. Neutrality was rejected for AgB2 genes. These data show the genetic polymorphism of antigen-coding genes among genetically characterized strains of E. granulosus.
Contido em Parasitology. Cambridge, Inglaterra. Vol. 131, n. 6 (Dec. 2005), p. 805-815
Assunto Antígeno B
Echinococcus granulosus
Polimorfismo genético
[en] Antigen B
[en] Echinococcus granulosus
[en] Genetic polymorphism
[en] RT-PCR
[en] SSCP
[en] Strains
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/27456
Arquivos Descrição Formato
000546262.pdf (136.5Kb) Texto completo Adobe PDF Visualizar/abrir

Este item está licenciado na Creative Commons License

Este item aparece na(s) seguinte(s) coleção(ões)


Mostrar registro completo

Percorrer



  • O autor é titular dos direitos autorais dos documentos disponíveis neste repositório e é vedada, nos termos da lei, a comercialização de qualquer espécie sem sua autorização prévia.
    Projeto gráfico elaborado pelo Caixola - Clube de Criação Fabico/UFRGS Powered by DSpace software, Version 1.8.1.