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Heterogeneity in extracellular nucleotide hydrolysis among clinical isolates of Trichomonas vaginalis

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Heterogeneity in extracellular nucleotide hydrolysis among clinical isolates of Trichomonas vaginalis

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Título Heterogeneity in extracellular nucleotide hydrolysis among clinical isolates of Trichomonas vaginalis
Autor Tasca, Tiana
Bonan, Carla Denise
De Carli, Geraldo Attilio
Sarkis, João José Freitas
Alderete, John Fernando
Abstract Trichomonas vaginalis is a parasitic protozoan that causes trichomonosis, a sexually-transmitted disease, with serious sequelae to women and men. As the host–parasite relationship is complex, it is important to investigate biochemical aspects of the parasite that contribute to our understanding of trichomonal biology and pathogenesis. Nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1), which hydrolyses extracellular ATP and ADP, and ecto-5k-nucleotidase, which hyrolyses AMP, have been characterized in laboratory isolates of T. vaginalis. Here we show that the extracellular ATP:ADP hydrolysis ratio varies among fresh clinical isolates, which presented higher ATPase and ADPase activities than long-term-grown isolates. Growth of parasites in iron-replete and iron-depleted medium resulted in different, albeit minor, patterns in extracellular ATP and ADP hydrolysis among isolates Importantly, some isolates had low or absent ecto-5k-nucleotidase activity, regardless of environmental conditions tested. For isolates with ecto-5k-nucleotidase activity, high- and low-iron trichomonads had increased and decreased levels of activity, respectively, compared to organisms grown in normal TYM-serum medium. This suggests a regulation in expression of either the enzyme amounts and/or activity under the control of iron. Finally, we found no correlation between the presence or absence of dsRNA virus infection among trichomonad isolates and NTPDase and ecto-5k-nucleotidase activities.
Contido em Parasitology. Cambridge, United Kingdom. Vol. 131, pt1 (2005), p. 71-78
Assunto Bioquímica
[en] Ecto-5k-nucleotidase
[en] Fresh isolates
[en] Heterogeneity
[en] Nucleoside triphosphate diphosphohydrolase
[en] Trichomonas vaginalis
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/27459
Arquivos Descrição Formato
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