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dc.contributor.authorTasca, Tianapt_BR
dc.contributor.authorBonan, Carla Denisept_BR
dc.contributor.authorDe Carli, Geraldo Attiliopt_BR
dc.contributor.authorSarkis, João José Freitaspt_BR
dc.contributor.authorAlderete, John Fernandopt_BR
dc.date.accessioned2011-01-21T05:59:01Zpt_BR
dc.date.issued2005pt_BR
dc.identifier.issn0031-1820pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/27459pt_BR
dc.description.abstractTrichomonas vaginalis is a parasitic protozoan that causes trichomonosis, a sexually-transmitted disease, with serious sequelae to women and men. As the host–parasite relationship is complex, it is important to investigate biochemical aspects of the parasite that contribute to our understanding of trichomonal biology and pathogenesis. Nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1), which hydrolyses extracellular ATP and ADP, and ecto-5k-nucleotidase, which hyrolyses AMP, have been characterized in laboratory isolates of T. vaginalis. Here we show that the extracellular ATP:ADP hydrolysis ratio varies among fresh clinical isolates, which presented higher ATPase and ADPase activities than long-term-grown isolates. Growth of parasites in iron-replete and iron-depleted medium resulted in different, albeit minor, patterns in extracellular ATP and ADP hydrolysis among isolates. Importantly, some isolates had low or absent ecto-5k-nucleotidase activity, regardless of environmental conditions tested. For isolates with ecto-5k-nucleotidase activity, high- and low-iron trichomonads had increased and decreased levels of activity, respectively, compared to organisms grown in normal TYM-serum medium. This suggests a regulation in expression of either the enzyme amounts and/or activity under the control of iron. Finally, we found no correlation between the presence or absence of dsRNA virus infection among trichomonad isolates and NTPDase and ecto-5k-nucleotidase activities.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofParasitology. London. Vol. 131, no. 1 (2005), p. 71-78pt_BR
dc.rightsOpen Accessen
dc.subjectTrichomonas vaginalisen
dc.subjectHidrólisept_BR
dc.subjectNucleoside triphosphate diphosphohydrolaseen
dc.subjectTrichomonas vaginalispt_BR
dc.subjectBioquímicapt_BR
dc.subjectEcto-5k-nucleotidaseen
dc.subjectFresh isolatesen
dc.subjectHeterogeneityen
dc.titleHeterogeneity in extracellular nucleotide hydrolysis among clinical isolates of Trichomonas vaginalispt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000530413pt_BR
dc.type.originEstrangeiropt_BR


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