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Localization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase

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Localization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase

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Título Localization and function of Rhipicephalus (Boophilus) microplus vitellin-degrading cysteine endopeptidase
Autor Seixas, Adriana
Estrela, Andreia Bergamo
Ceolato, Juliana Casagrande
Pontes, Emerson Guedes
Lara, Flávio Alves
Gondim, Katia Calp
Termignoni, Carlos
Abstract The tick Rhipicephalus (Boophilus) microplus is an important parasite of cattle in many areas of the tropics. Characterization of molecules involved in mechanisms such as vitellogenesis and embryo development may contribute to a better understanding of this parasite’s physiology. The vitellin-degrading cysteine endopeptidase (VTDCE) is the most active enzyme involved in vitellin hydrolysis in R. microplus eggs. Here we show an association between VTDCE and vitellin in an additional site, apart from the active site. Our data also demonstrate cysteine endopeptidase activity in different tissues such as ovary, gut, fat body, salivary gland and female haemolymph, where it is controlled by a physiological inhibitor. In R. microplus female gut, VTDCE is localized in areas of protein synthesis and trafficking with the underlying haemolymph. VTDCE is also localized in the ovary basal region, in vesicle membranes of ovary pedicel cells and in oocyte cytosol. These results suggest that VTDCE plays a role in vitellin digestion during tick development.
Contido em Parasitology. Cambridge, United Kingdom. Vol. 137, no. 12 (Oct. 2010), p. 1819-1831
Assunto Bioquímica
Carrapatos
Cisteína endopeptidases
Rhipicephalus microplus
[en] Cysteine endopeptidase
[en] Cysteine endopeptidase inhibitor
[en] Haemolymph
[en] Rhipicephalus (Boophilus) microplus
[en] Vitellin
[en] VTDCE
Origem Estrangeiro
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/27466
Arquivos Descrição Formato
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