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Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay

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Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay

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Título Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
Autor Maschmann, Raquel de Abreu
Verza, Mirela
Silva, Marcia Susana Nunes
Sperhacke, Rosa Dea
Ribeiro, Marta Osório
Suffys, Philip Noel
Gomes, Harrison Magdinier
Tortoli, Enrico
Marcelli, Fiorella
Zaha, Arnaldo
Rossetti, Maria Lucia Rosa
Abstract We used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis. When compared with the antimicrobial susceptibility test (AST), the sensitivity and specificity of the CRDH were 92.3% and 98.1%, respectively. When compared with sequencing, the sensitivity and specificity of the CRDH were 90.6% and 100%, respectively. To evaluate the performance of the assay directly in clinical specimens, 30 samples from tuberculosis patients were used. For these samples, the results of the CRDH were 100% consistent with the results of the AST and sequencing. These results indicate that the rate of concordance of the CRDH is high when compared to conventional methods and sequencing data. The CRDH can be successfully applied when a rapid test is required for the identification of RIF resistance in M. tuberculosis.
Contido em Memorias do Instituto Oswaldo Cruz : an International Journal of Biological and Biomedical Research. Rio de Janeiro. Vol. 106, n. 2 (mar. 2011), p. 139-145
Assunto Hibridização
Mycobacterium tuberculosis
Rifampicina
Tuberculose
[en] Drug resistance
[en] Renverse dot blot hybridization
[en] Rifampin
[en] rpoB
[en] Tuberculosis
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/30563
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