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The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate

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The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate

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Título The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate
Autor Cechetti, Fernanda
Worm, Paulo Valdeci
Silva, Lenir Orlandi Pereira
Siqueira, Ionara Rodrigues
Netto, Carlos Alexandre
Abstract Permanent bilateral occlusion of the common carotid arteries (2VO) in the rat has been established as a valid experimental model to investigate the effects of chronic cerebral hypoperfusion on cognitive function and neurodegenerative processes. Our aim was to compare the cognitive and morphological outcomes following the standard 2VO procedure, in which there is concomitant artery ligation, with those of a modified protocol, with a 1-week interval between artery occlusions to avoid an abrupt reduction of cerebral blood flow, as assessed by animal performance in the water maze and damage extension to the hippocampus and striatum. Male Wistar rats (N = 47) aged 3 months were subjected to chronic hypoperfusion by permanent bilateral ligation of the common carotid arteries using either the standard or the modified protocol, with the right carotid being the first to be occluded. Three months after the surgical procedure, rat performance in the water maze was assessed to investigate long-term effects on spatial learning and memory and their brains were processed in order to estimate hippocampal volume and striatal area. Both groups of hypoperfused rats showed deficits in reference (F(8,172) = 7.0951, P < 0.00001) and working spatial memory [2nd (F(2,44) = 7.6884, P < 0.001), 3rd (F(2,44) = 21.481, P < 0.00001) and 4th trials (F(2,44) = 28.620, P < 0.0001)]; however, no evidence of tissue atrophy was found in the brain structures studied. Despite similar behavioral and morphological outcomes, the rats submitted to the modified protocol showed a significant increase in survival rate, during the 3 months of the experiment (P < 0.02).
Contido em Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. São Paulo. Vol. 43, no. 12 (Dez. 2010), p. 1178-1183
Assunto Hipocampo
Hipoperfusão crônica
Memória
[en] Chronic cerebral hypoperfusion
[en] Hippocampus
[en] Spatial memory
[en] Striatum
[en] Water maze
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/30588
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