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Group B Streptococcus detection : comparison of PCR assay and culture as a screening method for pregnant women

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Group B Streptococcus detection : comparison of PCR assay and culture as a screening method for pregnant women

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Título Group B Streptococcus detection : comparison of PCR assay and culture as a screening method for pregnant women
Autor Paris, Fernanda de
Machado, Alice Beatriz Mombach Pinheiro
Gheno, Tailise Conte
Ascoli, Bruna Maria
Oliveira, Kátia Ruschel Pilger de
Barth, Afonso Luis
Abstract Streptococcus agalactiae or group B Streptococcus (GBS) is one of the most important causal agents of serious neonatal infections. Numerous assays have been evaluated for GBS screening in order to validate a fast and efficient method. The aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (PCR) with specific primers (atr gene). Two hundred and sixty-three samples were analyzed. Vaginal samples were collected, according to the Centers for Disease Control and Prevention (CDC) recommendations, from women over 35 weeks of pregnancy at Hospital de Clínicas de Porto Alegre (HCPA). Two different extraction methods were tested in all samples collected. PCR technique yielded 71 (26.99%) positive results. Sensitivity and specificity for PCR were 100% and 86.88%, respectively. PCR demonstrated a shorter turnaround time than the culture. The molecular methodology proved to be a useful screening for GBS, allowing effective treatment to be initiated in shorter time to prevent newborn infection.
Contido em Brazilian journal of infectious diseases. Salvador. Vol. 15, n. 4 (jul./ago. 2011), p. 323-327
Assunto Gravidez
Reação em cadeia da polimerase
Streptococcus agalactiae
[en] Culture
[en] Polymerase chain reaction
[en] Pregnancy
[en] Streptococcus agalactiae
Origem Nacional
Tipo Artigo de periódico
URI http://hdl.handle.net/10183/38143
Arquivos Descrição Formato
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