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dc.contributor.authorSchinke, Cláudiapt_BR
dc.contributor.authorGermani, Jose Carlospt_BR
dc.date.accessioned2012-06-28T01:32:31Zpt_BR
dc.date.issued2012pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/49843pt_BR
dc.description.abstractMacrophomina phaseolina, phylum Ascomycota, is a phytopathogenic fungus distributed worldwide in hot dry areas. There are few studies on its secreted lipases and none on its colony radial growth rate, an indicator of fungal ability to use nutrients for growth, on media other than potato-dextrose agar. In this study, 13 M. phaseolina isolates collected in different Brazilian regions were screened for fast-growth and the production of hydrolases of industrial interest, especially alkaline lipases. Hydrolase detection and growth rate determination were done on citric pectin, gelatin, casein, soluble starch, and olive oil as substrates. Ten isolates were found to be active on all substrates tested. The most commonly detected enzymes were pectinases, amylases, and lipases. The growth rate on pectin was significantly higher (P < 0.05), while the growth rates on the different media identified CMM 2105, CMM 1091, and PEL as the fastest-growing isolates. The lipase activity of four isolates grown on olive oil was followed for 4 days by measuring the activity in the cultivation broth. The specific lipolytic activity of isolate PEL was significantly higher at 96 h (130 mU mg protein–1). The broth was active at 37 °C, pH 8, indicating the potential utility of the lipases of this isolate in mild alkaline detergents. There was a strong and positive correlation (0.86) between radial growth rate and specific lipolytic activity.en
dc.format.mimetypeapplication/pdf
dc.language.isoengpt_BR
dc.relation.ispartofInternational microbiology. Barcelona. Vol. 15, (2012), p. 1-7pt_BR
dc.rightsOpen Accessen
dc.subjectPectinasesen
dc.subjectHidrolasespt_BR
dc.subjectMacrophomina phaseolinapt_BR
dc.subjectAmylasesen
dc.subjectProteasesen
dc.subjectFungospt_BR
dc.subjectLipolytic activityen
dc.subjectRadial growth rateen
dc.titleScreening brazilian macrophomina phaseolina isolates for alkaline lipases and other extracellular hydrolasespt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000835671pt_BR
dc.type.originEstrangeiropt_BR


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